SKU: 13-0045
{"num_reviews":0,"url":"https://epicypher-c9.mybigcommerce.com/products/antibodies/snap-chip-certified-antibodies/histone-h3k27ac-antibody-snap-chip-certified","add_this":[{"service":"facebook","annotation":""},{"service":"twitter","annotation":""},{"service":"google","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""}],"gtin":null,"id":765,"can_purchase":true,"meta_description":"Mouse monoclonal histone H3K27ac antibody rigorously tested for robust and reliable performance in ChIP assays. Also validated for ICC/IF.","category":["Epigenetics Kits and Reagents","Antibodies/PTM specific Antibodies","Antibodies/SNAP-ChIP<sup>®</sup> Certified Antibodies","Epigenetics Kits and Reagents/SNAP-ChIP® Certified Antibodies","Assays"],"AddThisServiceButtonMeta":"","main_image":null,"add_to_wishlist_url":"/wishlist.php?action=add&product_id=765","custom_fields":[{"id":"569","name":"Pack size","value":"50 µg"}],"sku":"13-0045","description":"<table border=\"0\" cellpadding=\"2\" cellspacing=\"2\" style=\"width: 100%\"> <tbody> <tr valign=\"top\"> <td> <table border=\"0\" cellpadding=\"1\" cellspacing=\"1\" style=\"width: 95%\"> <tbody> <tr> <td> <table border=\"0\" cellpadding=\"0\" cellspacing=\"0\" style=\"width: 100%\" > <tbody> <tr> <td align=\"left\" valign=\"top\"> <span style=\"font-family: arial, helvetica, sans-serif\" ><strong>Type: </strong>Monoclonal</span > </td> <td align=\"left\" valign=\"top\"> <span style=\"font-family: arial, helvetica, sans-serif\" ><strong>Host: </strong>Mouse</span > </td> </tr> <tr> <td align=\"left\" valign=\"top\"> <span style=\"font-family: arial, helvetica, sans-serif\" ><strong>Mol Wgt.: </strong>15 kDa</span > </td> <td align=\"left\" valign=\"top\"> <span style=\"font-family: arial, helvetica, sans-serif\" ><strong>Appl.: </strong>ChIP, ChIP-Seq, L, IF</span > </td> </tr> <tr> <td align=\"left\" valign=\"top\"> <span style=\"font-family: arial, helvetica, sans-serif\" ><strong>Format: </strong>Aff. Pur. IgG</span > </td> <td align=\"left\" valign=\"top\"> <span style=\"font-family: arial, helvetica, sans-serif\" ><strong>Reactivity: </strong>H, M, WR</span > </td> </tr> </tbody> </table> </td> </tr> <tr> <td valign=\"top\"> <span style=\"font-family: arial, helvetica, sans-serif\"> <strong> Histone H3K27ac Antibody, SNAP-ChIP Certified Description: </strong> This antibody meets EpiCypher’s “<a href=\"https://www.epicypher.com/technologies/snap-chip-spike-ins/certified-antibodies\">SNAP-ChIP<sup>®</sup> Certified</a>” criteria for specificity and efficient target enrichment in a ChIP experiment (<20% cross-reactivity across the panel, >5% recovery of target input). Histone H3 is one of the four proteins that are present in the nucleosome, the basic repeating subunit of chromatin, consisting of 147 base pairs of DNA wrapped around an octamer of core histone proteins (H2A, H2B, H3 and H4). This antibody reacts to H3K27ac and no cross reactivity with other lysine acylations in the EpiCypher SNAP-ChIP K-AcylStat panel, is detected. Antibody binding to H3K27ac in the context of phosphorylation at S28 (H3K27acS28ph) is inhibited to varying degrees in Luminex and ChIP (<i>Figures 1-2</i>). </span ><br /> </td> </tr> <tr> <td valign=\"top\"> <span style=\"font-family: arial, helvetica, sans-serif\"> <strong> Histone H3K27ac Antibody, SNAP-ChIP Certified Immunogen: </strong> A synthetic peptide corresponding to histone H3 acetylated at lysine 27. </span> <p></p> </td> </tr> <tr> <td valign=\"top\"> <span style=\"font-family: arial, helvetica, sans-serif\" ><strong >Histone H3K27ac Antibody, SNAP-ChIP Certified Formulation: </strong> Protein A affinity-purified antibody (1 mg/mL) in PBS pH 7.4, with 0.05% sodium azide.</span > <p></p> </td> </tr> <tr> <td valign=\"top\"> <span style=\"font-family: arial, helvetica, sans-serif\" ><strong >Histone H3K27ac Antibody, SNAP-ChIP Certified Storage and Stability: </strong> Stable for 1 year at -20°C from date of receipt.</span > <p></p> </td> </tr> <tr> <td valign=\"top\"> <span style=\"font-family: arial, helvetica, sans-serif\"> <strong> Histone H3K27ac Antibody, SNAP-ChIP Certified Application Notes </strong> <br /> <span style=\"font-weight: normal\"> <strong> Recommended dilutions: </strong> <br /> </span> <strong>ChIP/ChIP-seq</strong> : 2 - 5 μg per 1x10 <sup> 6 </sup> cells <br /> <strong> L </strong> : 1:4000 dilution <br /> <strong> IF </strong> : 1:500 </span> </td> </tr> <tr> <td valign=\"top\"> <p> <span style=\"font-family: arial, helvetica, sans-serif\"> <span style=\"font-size: 12pt\"> <strong >Histone H3K27ac Antibody, SNAP-ChIP Certified References: </strong> </span> </span> </p> <p></p> <p> <span style=\"font-family: arial, helvetica, sans-serif\" >Grzybowski et al (2015) Mol Cell 58:886 </span> </p> <p> <span style=\"font-family: arial, helvetica, sans-serif\"> Shah et al (2018) Mol Cell 72:162 </span> </p> </td> </tr> <tr> <td valign=\"top\"> <span style=\"font-family: arial, helvetica, sans-serif\" >View technical datasheet for this product. <a href=\"https://www.epicypher.com/content/documents/tds/13-0045.pdf\" target=\"_new\" > <img alt=\"13-0045 Datasheet\" height=\"40\" src=\"https://cdn7.bigcommerce.com/s-y9o92/content/documents/tds/icon.png\" width=\"30\" /></a ></span> </td> </tr> <tr> <td> <p></p> <p> <span style=\"font-family: arial, helvetica, sans-serif\" ><span style=\"font-size: 10pt\" ><strong>Applications Key: ChIP</strong>-Chromatin IP; <strong>E</strong>-ELISA; <strong>FACS</strong>-Flow cytometry; <strong>IF</strong>-Immunofluorescence; <strong>IHC</strong>-Immunohistochemistry; <strong>ICC</strong>-Immunocytochemistry; <strong>L</strong>-Luminex; <strong>IP</strong>-Immunoprecipitation; <strong>WB</strong>-Western Blotting</span ></span > </p> <p> <span style=\"font-family: arial, helvetica, sans-serif\" ><span style=\"font-size: 10pt\" ><strong>Reactivity Key: </strong ><strong>B</strong>-Bovine; <strong>Ce</strong>-C. elegans; <strong>Ch</strong>-Chicken; <strong>Dm</strong>- Drosophila; <strong>Eu</strong>-Eukaryote; <strong>H</strong>-Human; <strong>M</strong>-Mouse; <strong>Ma</strong>-Mammal; <strong>R</strong>-Rat; <strong>Sc</strong>-S.cerevesiae; <strong>Sp</strong>-S. pombe; <strong>WR</strong>-Wide Range (predicted); <strong>X</strong>-Xenopus; <strong>Z</strong>-Zebrafish</span ></span > </p> </td> </tr> </tbody> </table> </td> <td> <table border=\"0\" cellpadding=\"3\" cellspacing=\"3\" style=\"width: 300px\"> <tbody> <!--BEGIN IMAGE AND CAPTION SECTION--> <tr> <td align=\"center\"> <span style=\"font-family: arial, helvetica, sans-serif\" ><a href=\"https://cdn11.bigcommerce.com/s-y9o92/product_images/uploaded_images/h3k27ac-epicypher-13-0045.jpg?t=1600973784&_ga=2.162199103.241922123.1600715432-402741698.1598987696\" target=\"_new\" ><img alt=\"Representative SNAP-ChIP-seq results\" height=\"100\" src=\"https://cdn11.bigcommerce.com/s-y9o92/product_images/uploaded_images/h3k27ac-epicypher-13-0045.jpg?t=1600973784&_ga=2.162199103.241922123.1600715432-402741698.1598987696\" width=\"200\" /></a ></span> </td> </tr> <tr> <td align=\"left\" valign=\"top\"> <p> <span style=\"font-family: arial, helvetica, sans-serif\" ><strong>Representative SNAP-ChIP-seq results: </strong> Cumulative histogram plot and heatmap of signal intensity depict H3K27ac ChIP-seq data aligned to annotated transcription start sites (TSS, +/- 3.0 kb; left). Two representative genomic regions depicting H3K27ac peak structure and distribution are shown (right). Data shown are representative of H3K27ac ChIP antibody (EpiCypher Catalog No. 13-0045) and are not lot-specific. Native ChIP-seq was performed using K562 cells as described (<a href=\"https://pubmed.ncbi.nlm.nih.gov/30244833/\" target=\"_blank\" >Shah et al., Mol Cell 2018</a >) with SNAP-ChIP<sup>TM</sup> K-AcylStat Spike-in (Catalog No. <a href=\"https://www.epicypher.com/products/nucleosomes/snap-chip-k-acylstat-panel\" target=\"_blank\" >19-3001</a >) nucleosome controls added prior to chromatin digestion to confirm antibody specificity and ChIP efficiency. Paired-end sequencing libraries were prepared using the NEBNext<sup>®</sup> Ultra<sup>TM</sup> II DNA Library Prep Kit for Illumina<sup>®</sup>. ChIP libraries were sequenced on an Illumina<sup>®</sup> NextSeq. Sequencing reads were aligned to the human genome using Bowtie 2 (Johns Hopkins University). Bigwig files of read enrichment in binned genomic regions (signal intensity) flanking the indicated gene features were used to create a cumulative histogram plot and heatmap of signal intensity (<a href=\"https://basepairtech.com/\" target=\"_blank\"> www.basepairtech.com</a >). Gene browser shots were generated using the Integrative Genomics Viewer (IGV, Broad Institute) with the window size denoted (top) <br /> <strong>(Click image to enlarge) </strong></span > </p> <p></p> </td> </tr> <tr> <td align=\"center\"> <span style=\"font-family: arial, helvetica, sans-serif\" ><a href=\"https://www.epicypher.com/content/images/products/antibodies/13-0045_luminex.jpg\" target=\"_new\" ><img alt=\"13-0045 Luminex Data\" height=\"100\" src=\"https://cdn11.bigcommerce.com/s-y9o92/content/images/products/antibodies/13-0045_luminex.jpg\" width=\"200\" /></a ></span> </td> </tr> <tr> <td align=\"left\" valign=\"top\"> <p> <span style=\"font-family: arial, helvetica, sans-serif\" ><strong>Luminex Data:</strong> Histone H3K27ac antibody was assessed using a Luminex<sup>®</sup> based approach employing dCypher™ Nucleosome K-AcylStat Panel (EpiCypher Catalog No. 16-9003). The panel comprises biotinylated designer nucleosomes (X-Axis) individually coupled to uniquely identifiable Luminex Magplex<sup>®</sup> beads. Antibody binding to nucleosomes was tested in multiplex (23-plex) at a 1:4000 dilution, and detected with second layer anti-IgG*PE. Data was generated using a Luminex FlexMAP3D<sup>®</sup>. Data normalized to relevant on-target (H3K27ac; set to 100) is shown. <br /> <strong>(Click image to enlarge) </strong></span > </p> <p></p> </td> </tr> <!--END IMAGE AND CAPTION SECTION--> <tr valign=\"top\"> <td align=\"center\" valign=\"top\"> <span style=\"font-family: arial, helvetica, sans-serif\" ><a href=\"https://www.epicypher.com/content/images/products/antibodies/13-0045_SNAP_qPCR.jpg\" target=\"_new\" ><img alt=\"13-0045 SNAP-ChIP qPCR Data\" height=\"100\" src=\"https://cdn11.bigcommerce.com/s-y9o92/content/images/products/antibodies/13-0045_SNAP_qPCR.jpg\" width=\"350\" /></a ></span> </td> </tr> <tr> <td align=\"left\" valign=\"top\"> <p> <span style=\"font-family: arial, helvetica, sans-serif\" ><strong>SNAP-ChIP-qPCR Data:</strong> Histone H3K27ac antibody (3 µg) was tested in a native ChIP experiment using chromatin from K-562 cells (3 µg) with the SNAP-ChIP K-AcylStat Panel (EpiCypher Catalog No. 19-3001) spiked-in prior to micrococcal nuclease digestion. Specificity (left Y-axis) was determined by qPCR for the DNA barcodes corresponding to modified nucleosomes in the SNAP-ChIP panel (x-axis). Black bar represents antibody efficiency (right y-axis; log scale) and indicates percentage of the target immunoprecipitated relative to input. Error bars represent mean ± SEM in replicate ChIP experiments. <br /> <strong>(Click image to enlarge) </strong></span > </p> <p></p> </td> </tr> <tr valign=\"top\"> <td align=\"center\" valign=\"top\"> <span style=\"font-family: arial, helvetica, sans-serif\" ><a href=\"https://www.epicypher.com/content/images/products/antibodies/13-0045_IF.jpg\" target=\"_new\" ><img alt=\"13-0045 Immunofluorescence\" height=\"100\" src=\"https://cdn11.bigcommerce.com/s-y9o92/content/images/products/antibodies/13-0045_IF.jpg\" width=\"127\" /></a ></span> </td> </tr> <tr> <td align=\"left\" valign=\"top\"> <p> <span style=\"font-family: arial, helvetica, sans-serif\" ><strong>Immunofluorescence Data: </strong> IF detection of H3K27ac in HeLa cells stained with H3K27ac antibody at a dilution of 1:500, followed by the addition of an anti-mouse secondary antibody conjugated to a fluorophore (left). The middle pannel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.<br /> <strong>(Click image to enlarge) </strong></span > </p> <p></p> </td> </tr> </tbody> </table> </td> </tr> </tbody> </table>","tags":[],"warranty":"","price":{"without_tax":{"formatted":"$515.00","value":515,"currency":"USD"},"tax_label":"Tax"},"detail_messages":"","availability":"","page_title":"H3K27ac Antibody | SNAP-ChIP® Certified","cart_url":"https://epicypher-c9.mybigcommerce.com/cart.php","max_purchase_quantity":0,"mpn":null,"upc":null,"options":[{"id":141,"type":"Configurable_PickList_Set","display_name":"#1","required":true,"state":"variant_option","partial":"set-select","condition":true,"values":[{"id":110,"label":"abc","selected":false,"data":"abc"},{"id":111,"label":"xyz","selected":false,"data":"xyz"}]},{"id":142,"type":"Configurable_PickList_Set","display_name":"#2","required":true,"state":"variant_option","partial":"set-select","condition":true,"values":[{"id":117,"label":"abc","selected":false,"data":"abc"},{"id":118,"label":"xyz","selected":false,"data":"xyz"}]}],"related_products":[{"id":709,"sku":"13-0037","name":"Histone H4K12ac Antibody - 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Pack size: 50 µg
