SKU: 16-0002
{"num_reviews":0,"url":"https://epicypher-c9.mybigcommerce.com/products/nucleosomes/hela-mononucleosomes-purified","add_this":[{"service":"facebook","annotation":""},{"service":"twitter","annotation":""},{"service":"google","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""}],"gtin":null,"id":129,"can_purchase":true,"meta_description":"Purified HeLa Mononucleosomes for enzyme screening assays and chromatin binding experiments","category":["Nucleosomes","Nucleosomes/Purified Nucleosomes (HeLa, Avian)"],"AddThisServiceButtonMeta":"","main_image":null,"add_to_wishlist_url":"/wishlist.php?action=add&product_id=129","custom_fields":[{"id":"13","name":"Pack Size","value":"50 μg"}],"sku":"16-0002","description":"<div class=\"product-general-info\"> <ul class=\"product-general-info__list-left\"> <li class=\"product-general-info__list-item\"> <strong>Species: </strong>Human </li> <li class=\"product-general-info__list-item\"> <strong>Source: </strong>HeLa cells </li> </ul> <ul class=\"product-general-info__list-right\"> <li class=\"product-general-info__list-item\"> <strong>Tag: </strong>N/A </li> <li class=\"product-general-info__list-item\"> <strong>Molecular Weight: </strong>214,000 Da </li> </ul> </div> <div class=\"service_accordion product-droppdown\"> <div class=\"container\"> <div id=\"prodAccordion\"> <div id=\"ProductDescription\" class=\"Block Panel current\"> <h3 class=\"sub-title1\">Description</h3> <div class=\"ProductDescriptionContainer product-droppdown__section-description-specific\"> <p> Human mononucleosomes purified from HeLa cells. The nucleosome is the basic repeating unit of chromatin, which consists of 150 base pairs of DNA wrapped around an octamer core of histone proteins (two each of H2A, H2B, H3, and H4). </p> </div> </div> </div> <div id=\"prodAccordion\"> <div id=\"ProductDescription\" class=\"Block Panel current\"> <h3 class=\"sub-title1\">Validation Data</h3> <div class=\"ProductDescriptionContainer product-droppdown__section-description-specific\"> <section class=\"image-picker\"> <div class=\"image-picker__left\"> <div class=\"image-picker__main-content_active image-picker__main-content\"> <div class=\"image-picker__header-content\"> <button class=\"image-picker__left-arrow\"> <svg class=\"image-picker__svg-left\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\"> <path d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" /> </svg> </button> <a href=\"/content/images/products/nucleosomes/16-0002-dna-gel-data.jpeg\" target=\"_blank\" class=\"image-picker__main-image-link\" ><img alt=\"16-0002-dna-gel-data\" src=\"/content/images/products/nucleosomes/16-0002-dna-gel-data.jpeg\" class=\"image-picker__main-image\" /> <span class=\"image-picker__main-image-caption\" >(Click to enlarge)</span ></a > <button class=\"image-picker__right-arrow\"> <svg class=\"image-picker__svg-right\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\"> <path d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" /> </svg> </button> </div> <p> <span class=\"image-picker__span-content\" ><strong>Figure 1: DNA gel gata </strong><br /> DNA (2 µg) was purified from HeLa Mononucleosomes and resolved via agarose gel to show the size of nucleosomal DNA compared to molecular weight markers (base pairs). Mononucleosomal DNA resolves at ~150 base pairs. </span> </p> </div> <div class=\"image-picker__main-content\"> <div class=\"image-picker__header-content\"> <button class=\"image-picker__left-arrow\"> <svg class=\"image-picker__svg-left\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\"> <path d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" /> </svg> </button> <a href=\"/content/images/products/nucleosomes/16-0002-protein-gel-data.jpeg\" target=\"_blank\" class=\"image-picker__main-image-link\" ><img alt=\"16-0002-protein-gel-data\" src=\"/content/images/products/nucleosomes/16-0002-protein-gel-data.jpeg\" class=\"image-picker__main-image\" /> <span class=\"image-picker__main-image-caption\" >(Click to enlarge)</span ></a > <button class=\"image-picker__right-arrow\"> <svg class=\"image-picker__svg-right\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\"> <path d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" /> </svg> </button> </div> <p> <span class=\"image-picker__span-content\" ><strong>Figure 2: Protein gel data </strong><br /> Coomassie stained SDS-PAGE gel of proteins in HeLa Mononucleosomes (2 µg) demonstrates the purity of histones in the preparation. Sizes of molecular weight markers and position of the core histones (H2A, H2B, H3, and H4) are indicated. </span> </p> </div> <div class=\"image-picker__main-content\"> <div class=\"image-picker__header-content\"> <button class=\"image-picker__left-arrow\"> <svg class=\"image-picker__svg-left\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\"> <path d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" /> </svg> </button> <a href=\"/content/images/products/nucleosomes/16-0002-enzyme-activity-data.jpeg\" target=\"_blank\" class=\"image-picker__main-image-link\"> <img alt=\"16-0002-enzyme-activity-data\" src=\"/content/images/products/nucleosomes/16-0002-enzyme-activity-data.jpeg\" class=\"image-picker__main-image\" /> <span class=\"image-picker__main-image-caption\" >(Click to enlarge)</span > </a> <button class=\"image-picker__right-arrow\"> <svg class=\"image-picker__svg-right\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\"> <path d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" /> </svg> </button> </div> <p> <span class=\"image-picker__span-content\"> <strong>Figure 3: Enzyme activity data </strong><br /> HMTase assay with HeLa Mononucleosomes (1 µg) and SET8 (HeLa Nucs + SET8; 1 µg) using a standard radiometric filter binding assay protocol. Controls include the mononucleosomes alone (HeLa Nucs) or SET8 alone (SET8). SET8 is aa 1-352 of UniProt# Q9NQR1. </span> </p> </div> </div> <aside class=\"image-picker__right\"> <div class=\"image-picker__gallery\"> <img alt=\"16-0002-dna-gel-data\" src=\"/content/images/products/nucleosomes/16-0002-dna-gel-data.jpeg\" width=\"200\" class=\"image-picker__side-image image-picker__side-image_active\" role=\"button\" /> <img alt=\"16-0002-protein-gel-data\" src=\"/content/images/products/nucleosomes/16-0002-protein-gel-data.jpeg\" class=\"image-picker__side-image\" role=\"button\" /> <img alt=\"16-0002-enzyme-activity-data\" src=\"/content/images/products/nucleosomes/16-0002-enzyme-activity-data.jpeg\" class=\"image-picker__side-image\" role=\"button\" /> </div> </aside> </section> </div> </div> </div> <div id=\"prodAccordion\"> <div id=\"ProductDescription\" class=\"Block Panel\"> <h3 class=\"sub-title1\">Technical Information</h3> <div class=\"ProductDescriptionContainer product-droppdown__section-description\"> <div class=\"product-tech-info\"> <div class=\"product-tech-info__line-item\"> <div class=\"product-tech-info__line-item-left\"> <b>Storage</b> </div> <div class=\"product-tech-info__line-item-right\"> Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid freeze/thaws </div> </div> <div class=\"product-tech-info__line-item\"> <div class=\"product-tech-info__line-item-left\"> <b>Formulation</b> </div> <div class=\"product-tech-info__line-item-right\"> 20 mM HEPES pH 7.5, 1 mM EDTA (26.9 µg protein, 50 µg DNA + protein) </div> </div> </div> </div> </div> </div> <div id=\"prodAccordion\"> <div id=\"ProductDescription\" class=\"Block Panel\"> <h3 class=\"sub-title1\">Application Notes</h3> <div class=\"ProductDescriptionContainer product-droppdown__section-description\"> <p> HeLa Mononucleosomes are suitable for use in enzyme assays such as acetylation or methylation, chromatin binding, or as a positive control in western blotting. Use 1-2 µg per reaction. </p> <p> Molarity and molecular weight are estimated based on DNA size and account for the endogenous post-translational histone modifications. </p> <p> Note: despite the high purity of the preparation, a small amount of arginine methyltransferase (RMTase) activity may co-purify with the nucleosomes. If you are studying weak or low turnover HMTases, you can use AMI-1 to inhibit the endogenous RMTase activity. </p> </div> </div> </div> <div id=\"prodAccordion\"> <div id=\"ProductDescription\" class=\"Block Panel\"> <h3 class=\"sub-title1\">Gene & Protein Information</h3> <div class=\"ProductDescriptionContainer product-droppdown__section-description\"> <div class=\"product-tech-info\"> <div class=\"product-tech-info__line-item\"> <div class=\"product-tech-info__line-item-left\"> <b>UniProt ID</b> </div> <div class=\"product-tech-info__line-item-right\"> H2A - P04908 (alt. names: H2A type 1-B/E, H2A.2, H2A/a, H2A/m)<br /> H2B - O60814 (alt. names: H2B K, HIRA-interacting protein 1)<br /> H3 - P68431, Q71DI3, K7EMV3<br /> H4 - P62805 </div> </div> </div> </div> </div> </div> <div id=\"prodAccordion\"> <div id=\"ProductDescription\" class=\"Block Panel\"> <h3 class=\"sub-title1\">References</h3> <div class=\"ProductDescriptionContainer product-droppdown__section-description\"> <strong>Background References:</strong> <br /> [1] Kuo et al. <em>Mol. Cell</em> (2011). PMID: <a href=\"https://pubmed.ncbi.nlm.nih.gov/22099308/\" target=\"_blank\" title=\"NSD2 links dimethylation of histone H3 at lysine 36 to oncogenic programming\" >22099308</a ><br /> [2] Matthews et al. <em>Nature</em> (2007). PMID: <a href=\"https://pubmed.ncbi.nlm.nih.gov/18033247/\" target=\"_blank\" title=\"RAG2 PHD finger couples histone H3 lysine 4 trimethylation with V(D)J recombination\" >18033247</a ><br /> [3] Shi et al. <em>Nature</em> (2006). PMID: <a href=\"https://pubmed.ncbi.nlm.nih.gov/16728974/\" target=\"_blank\" title=\"ING2 PHD domain links histone H3 lysine 4 methylation to active gene repression\" >16728974</a ><br /> </div> </div> </div> <div id=\"prodAccordion\"> <div id=\"ProductDescription\" class=\"Block Panel\"> <h3 class=\"sub-title1\">Documents & Resources</h3> <div class=\"ProductDescriptionContainer product-droppdown__section-description\"> <div class=\"product-documents\"> <a href=\"/content/documents/tds/16-0002.pdf\" target=\"_blank\" class=\"product-documents__link\"> <svg version=\"1.1\" id=\"Layer_1\" xmlns=\"http://www.w3.org/2000/svg\" xmlns:xlink=\"http://www.w3.org/1999/xlink\" x=\"0px\" y=\"0px\" viewBox=\"0 0 228 240\" style=\"enable-background: new 0 0 228 240\" xml:space=\"preserve\" class=\"product-documents__icon\" alt=\"16-0002 Datasheet\"> <g> <path class=\"product-documents__svg-pdf\" d=\"M191.92,68.77l-47.69-47.69c-1.33-1.33-3.12-2.08-5.01-2.08H45.09C41.17,19,38,22.17,38,26.09v184.36 c0,3.92,3.17,7.09,7.09,7.09h141.82c3.92,0,7.09-3.17,7.09-7.09V73.8C194,71.92,193.25,70.1,191.92,68.77z M177.65,77.06h-41.7 v-41.7L177.65,77.06z M178.05,201.59H53.95V34.95h66.92v47.86c0,5.14,4.17,9.31,9.31,9.31h47.86V201.59z\" /> </g> <rect x=\"20\" y=\"112\" class=\"product-documents__svg-background\" width=\"146\" height=\"76\" /> <g> <path class=\"product-documents__svg-pdf\" d=\"M23.83,125.68h22.36c5.29,0,9.41,1.33,12.35,4c2.94,2.67,4.42,6.39,4.42,11.18c0,4.78-1.47,8.51-4.42,11.18 c-2.94,2.67-7.06,4-12.35,4H34.59v18.29H23.83V125.68z M44.81,147.9c5.38,0,8.07-2.32,8.07-6.97c0-2.39-0.67-4.16-2-5.31 c-1.33-1.15-3.36-1.73-6.07-1.73H34.59v14.01H44.81z\" /> <path class=\"product-documents__svg-pdf\" d=\"M69.92,125.68h18.91c5.29,0,9.84,0.97,13.66,2.9c3.82,1.93,6.74,4.72,8.76,8.35 c2.02,3.63,3.04,7.98,3.04,13.04c0,5.06-1,9.42-3,13.08c-2,3.66-4.91,6.45-8.73,8.38c-3.82,1.93-8.4,2.9-13.73,2.9H69.92V125.68z M88.07,165.63c10.35,0,15.52-5.22,15.52-15.66c0-10.4-5.17-15.59-15.52-15.59h-7.38v31.26H88.07z\" /> <path class=\"product-documents__svg-pdf\" d=\"M122.57,125.68h32.84v8.49h-22.22v11.18h20.84v8.49h-20.84v20.49h-10.63V125.68z\" /> </g> </svg> <span class=\"product-documents__info\">Technical Datasheet</span> </a> </div> </div> </div> </div> </div> </div>","tags":[],"warranty":"","price":{"without_tax":{"formatted":"$495.00","value":495,"currency":"USD"},"tax_label":"Tax"},"detail_messages":"","availability":"","page_title":"Purified HeLa Mononucleosomes","cart_url":"https://epicypher-c9.mybigcommerce.com/cart.php","max_purchase_quantity":0,"mpn":null,"upc":"50 µg","options":[],"related_products":[{"id":133,"sku":"16-0003","name":"HeLa Polynucleosomes, Purified","url":"https://epicypher-c9.mybigcommerce.com/products/nucleosomes/hela-polynucleosomes-purified","availability":"","rating":0,"brand":{"name":null},"category":["Nucleosomes","Nucleosomes/Purified Nucleosomes (HeLa, Avian)"],"summary":" Species: Human Source: HeLa cells ","image":null,"images":[],"date_added":"18th Jan 2024","pre_order":false,"show_cart_action":true,"has_options":false,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":18,"name":"Pack Size","value":"50 μg"},{"id":19,"name":"Internal Comment","value":"has bulk in LG"}],"num_reviews":0,"weight":{"formatted":"0.01 Ounces","value":0.01},"demo":false,"add_to_cart_url":"https://epicypher-c9.mybigcommerce.com/cart.php?action=add&product_id=133","price":{"without_tax":{"currency":"USD","formatted":"$320.00","value":320},"tax_label":"Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=133"},{"id":495,"sku":"16-0019","name":"Chicken Mononucleosomes, Purified","url":"https://epicypher-c9.mybigcommerce.com/products/nucleosomes/chicken-mononucleosomes-purified","availability":"","rating":0,"brand":{"name":null},"category":["Nucleosomes","Nucleosomes/Purified Nucleosomes (HeLa, Avian)"],"summary":" Chicken Mononucleosomes, Purified Description: Mononucleosomes purified from chicken erythro","image":null,"images":[],"date_added":"18th Jan 2024","pre_order":false,"show_cart_action":true,"has_options":false,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":336,"name":"Pack Size","value":"50 μg"}],"num_reviews":0,"weight":{"formatted":"0.01 Ounces","value":0.01},"demo":false,"add_to_cart_url":"https://epicypher-c9.mybigcommerce.com/cart.php?action=add&product_id=495","price":{"without_tax":{"currency":"USD","formatted":"$215.00","value":215},"tax_label":"Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=495"},{"id":122,"sku":"17-0001","name":"HeLa Nuclear Extract","url":"https://epicypher-c9.mybigcommerce.com/products/epigenetics-kits-and-reagents/hela-nuclear-extract","availability":"","rating":0,"brand":{"name":null},"category":["Epigenetics Kits and Reagents","Epigenetics Kits and Reagents/Cell Extracts"],"summary":" HeLa Nuclear Extract Description: HeLa cells are an immortalized human cell line derived from a cervical cancer biopsy. 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Pack Size: 50 μg
