SKU: 16-0008
{"num_reviews":0,"url":"https://epicypher-c9.mybigcommerce.com/products/nucleosomes/histone-h3-h4-tetramer-recombinant-human","add_this":[{"service":"facebook","annotation":""},{"service":"twitter","annotation":""},{"service":"google","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""}],"gtin":null,"id":275,"can_purchase":true,"meta_description":"Histone H3/H4 Tetramer, Recombinant Human Protein for Enzyme Screening Assays","category":["Nucleosomes","Recombinant Proteins/Recombinant Histones","Nucleosomes/Nucleosome Components and Subunits"],"AddThisServiceButtonMeta":"","main_image":null,"add_to_wishlist_url":"/wishlist.php?action=add&product_id=275","custom_fields":[{"id":"153","name":"Pack Size","value":"50 μg"},{"id":"154","name":"Internal Comment","value":"Has Bulk in LG"}],"sku":"16-0008","description":"<div><table width=\"100%\" border=\"0\" cellspacing=\"2\" cellpadding=\"2\"> <tbody> <tr valign=\"top\"> <td> <table width=\"95%\" border=\"0\" cellspacing=\"1\" cellpadding=\"1\"> <tbody> <tr> <td> <table width=\"100%\" border=\"0\" cellspacing=\"0\" cellpadding=\"0\"> <tbody> <tr> <td align=\"left\" valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><b>Type: </b>Histone</span></span> </td> <td align=\"left\" valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><b>Host: </b><em>E. coli</em></span></span> </td> </tr> <tr> <td align=\"left\" valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><b>Mol Wgt.: </b>54 kDa</span></span> </td> <td align=\"left\" valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><b>Epitope Tag: </b>by product</span></span> </td> </tr> </tbody> </table> </td> </tr> <tr> <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><br /> <b>Histone H3/H4 Tetramers, Recombinant Human Description:</b> Histone H3/H4 Tetramers made from recombinant histones expressed in <em>E. coli</em> (accession numbers: H3-P68431; H4-P62805). Histones H3 and H4 were expressed and purified individually by FPLC, then assembled into tetramers that were further purified using gel filtration chromatography.<br /> <br /> </span></span> </td> </tr> <tr> <td valign=\"top\"> <span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b>Histone H3/H4 Tetramers, Recombinant Human Formulation:</b> 50 µg of H3/H4 Tetramer (1 mg/ml) in 10mM Tris-HCl pH 7.5, 2M NaCl, 1mM EDTA, 2mM DTT, 20% glycerol.<br /> <br /> </span></span> </td> </tr> <tr> <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b>Histone H3/H4 Tetramers, Recombinant Human Storage and Stability:</b> Lyophilized proteins are stable for 2 years at -20°C from date of shipment. Reconstitute with sterile distilled water and aliquot to avoid repeated freezing and thawing. <span class=\"Apple-style-span\" style=\"font-size: 12pt; \"> Stable for 6 months at -80°C <span class=\"Apple-style-span\" style=\"font-size: 12pt; \">after reconstitution.</span></span><br /> <br /> </span></span> </td> </tr> <tr> <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b>Histone H3/H4 Tetramers, Recombinant Human Application Notes:</b> Recombinant histone H3/H4 Tetramers are suitable for enzyme assays and nucleosome reconstitution.<br /> </span></span> </td> </tr> <tr> <td valign=\"top\"> <p><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b>References:</b><br /> <br /> </span></span><br /> </p> </td> </tr> <tr> <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\">View technical datasheet for this product. <a href=\"/content/documents/tds/16-0008.pdf\" target=\"_new\"> <img src=\"/content/documents/tds/icon.png\" alt=\"16-0008 Datasheet\" width=\"30\" height=\"40\" /></a></span></span> </td> </tr> </tbody> </table> </td> <td> <table width=\"100%\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\"> <tbody> <tr> <td align=\"center\"><a href=\"/content/images/products/proteins/16_0008_gel_tds.jpg\" target=\"_new\"> <img src=\"/content/images/products/proteins/16_0008_gel_tds.jpg\" alt=\"16-0008 Gel and Purity QC Data\" width=\"114\" height=\"250\" /></a> </td> </tr> <tr> <td align=\"left\" valign=\"top\"> <p><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><strong>QC Protein Data:</strong> Histone H3/H4 Tetramers, Recombinant Human, (1 µg) was run on a PAGE gel and stained with Coomassie blue. The migration and molecular weight of the protein standards are indicated.<br /> <strong>(Click image to enlarge) </strong></span></span></p> </td> </tr> <tr> <td align=\"center\"><a href=\"/content/images/products/proteins/16_0008_chrom_tds.jpg\" target=\"_new\"> <img src=\"/content/images/products/proteins/16_0008_chrom_tds.jpg\" alt=\"16-0008 Chromatofram Purity QC Data\" width=\"200\"/></a> </td> </tr> <tr> <td align=\"left\" valign=\"top\"> <p><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><strong>QC Purification Data:</strong> Chromatogram from gel filtration purification of Histone H3/H4 Tetramers, Recombinant Human. Only fractions corresponding to the intact tetramer (as indicated, black bar) were collected and pooled.<br /> <strong>(Click image to enlarge) </strong></span></span></p> </td> </tr> </tbody> </table> </td> </tr> </tbody></table> </div>","tags":[],"warranty":"","price":{"without_tax":{"formatted":"$160.00","value":160,"currency":"USD"},"tax_label":"Tax"},"detail_messages":"","availability":"","page_title":"Histone H3/H4 Tetramer, Recombinant Human Protein","cart_url":"https://epicypher-c9.mybigcommerce.com/cart.php","max_purchase_quantity":0,"mpn":null,"upc":null,"options":[],"related_products":[{"id":292,"sku":"16-0010","name":"Histone CENP-A / H4 Tetramer, Recombinant Human","url":"https://epicypher-c9.mybigcommerce.com/products/nucleosomes/histone-cenp-a-h4-tetramer-recombinant-human","availability":"","rating":0,"brand":{"name":null},"category":["Nucleosomes","Recombinant Proteins/Recombinant Histones","Nucleosomes/Nucleosome Components and Subunits"],"summary":" \t \t\t \t\t\t 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Pack Size: 50 μg
