SKU: 18-0005
{"num_reviews":0,"url":"https://epicypher-c9.mybigcommerce.com/products/nucleosomes/nucleosome-assembly-601-sequence-dna-biotinylated","add_this":[{"service":"facebook","annotation":""},{"service":"twitter","annotation":""},{"service":"google","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""}],"gtin":null,"id":138,"can_purchase":true,"meta_description":"Nucleosome Assembly 601 Sequence DNA, Biotinylated for chromatin assembly and nucleosome reconstitution, useful for pull-down and binding assays","category":["Nucleosomes","Nucleosomes/Nucleosome Components and Subunits"],"AddThisServiceButtonMeta":"","main_image":null,"add_to_wishlist_url":"/wishlist.php?action=add&product_id=138","custom_fields":[{"id":"24","name":"Pack Size","value":"50 μg"}],"sku":"18-0005","description":"<div class=\"service_accordion product-droppdown\"> <div class=\"container\"> <div id=\"prodAccordion\"> <div id=\"ProductDescription\" class=\"Block Panel current\"> <h3 class=\"sub-title1\">Description</h3> <div class=\"ProductDescriptionContainer product-droppdown__section-description-specific\"> <p> Nucleosome Assembly 601 Sequence DNA is a 147 base-pair double-stranded DNA fragment that was identified by Lowary and Widom using the SELEX method [1]. The 601 Sequence DNA has high affinity for histone octamers and is useful for <em>in vitro</em> nucleosome assembly. The DNA contains a 5’ biotin-TEG group. </p> </div> </div> </div> <div id=\"prodAccordion\"> <div id=\"ProductDescription\" class=\"Block Panel current\"> <h3 class=\"sub-title1\">Validation Data</h3> <div class=\"ProductDescriptionContainer product-droppdown__section-description-specific\"> <section class=\"image-picker\"> <div class=\"image-picker__left\"> <div class=\"image-picker__main-content_active image-picker__main-content\"> <div class=\"image-picker__header-content\"> <button class=\"image-picker__left-arrow\"> <svg class=\"image-picker__svg-left\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\"> <path d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" /> </svg> </button> <a href=\"/content/images/products/nucleosomes/18-0005-dna-gel-data.jpeg\" target=\"_blank\" class=\"image-picker__main-image-link\" ><img alt=\"18-0005-dna-gel-data\" src=\"/content/images/products/nucleosomes/18-0005-dna-gel-data.jpeg\" class=\"image-picker__main-image\" /> <span class=\"image-picker__main-image-caption\" >(Click to enlarge)</span ></a > <button class=\"image-picker__right-arrow\"> <svg class=\"image-picker__svg-right\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\"> <path d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" /> </svg> </button> </div> <p> <span class=\"image-picker__span-content\" ><strong>Figure 1: DNA Gel Data </strong><br /> Nucleosome Assembly 601 Sequence DNA, Biotinylated (75 ng) resolved via native PAGE gel and stained with ethidium bromide to visualize DNA. Migration positions of DNA molecular weight markers are indicated. </span> </p> </div> </div> <aside class=\"image-picker__right\"> <div class=\"image-picker__gallery\"> <img alt=\"18-0005-dna-gel-data\" src=\"/content/images/products/nucleosomes/18-0005-dna-gel-data.jpeg\" width=\"200\" class=\"image-picker__side-image image-picker__side-image_active\" role=\"button\" /> </div> </aside> </section> </div> </div> </div> <div id=\"prodAccordion\"> <div id=\"ProductDescription\" class=\"Block Panel\"> <h3 class=\"sub-title1\">Technical Information</h3> <div class=\"ProductDescriptionContainer product-droppdown__section-description\"> <div class=\"product-tech-info\"> <div class=\"product-tech-info__line-item\"> <div class=\"product-tech-info__line-item-left\"> <b>Storage</b> </div> <div class=\"product-tech-info__line-item-right\"> Stable for 2 years at -20°C from date of receipt. After resuspending, aliquots should be stored at -80°C. </div> </div> <div class=\"product-tech-info__line-item\"> <div class=\"product-tech-info__line-item-left\"> <b>Formulation</b> </div> <div class=\"product-tech-info__line-item-right\"> 50 µg lyophilized 601 Sequence DNA </div> </div> </div> </div> </div> </div> <div id=\"prodAccordion\"> <div id=\"ProductDescription\" class=\"Block Panel\"> <h3 class=\"sub-title1\">Application Notes</h3> <div class=\"ProductDescriptionContainer product-droppdown__section-description\"> <p> Nucleosome Assembly 601 Sequence DNA is useful for assembly of nucleosomes using purified or recombinant histone octamers (EpiCypher <a href=\"https://www.epicypher.com/products/nucleosomes/histone-octamer-recombinant-human\" >16-0001</a >). <a href=\"https://pubmed.ncbi.nlm.nih.gov/10804500/\"> See Luger et al. </a> [2] for recommended nucleosome reconstitution protocol. The biotin group on the DNA allows for pull-down of the nucleosomes for nucleosome binding experiments or enzymatic assays. </p> </div> </div> </div> <div id=\"prodAccordion\"> <div id=\"ProductDescription\" class=\"Block Panel\"> <h3 class=\"sub-title1\">References</h3> <div class=\"ProductDescriptionContainer product-droppdown__section-description\"> <strong>Background References:</strong> <br /> [1] Lowary & Widom <em>J. Mol. Biol.</em> (1998). PMID: <a href=\"https://pubmed.ncbi.nlm.nih.gov/9514715/\" target=\"_blank\" title=\"New DNA sequence rules for high affinity binding to histone octamer and sequence-directed nucleosome positioning\" >9514715</a ><br /> [2] Luger et al. <em>Methods Mol. Biol..</em> (1999). PMID: <a href=\"https://pubmed.ncbi.nlm.nih.gov/10804500/\" target=\"_blank\" title=\"Expression and purification of recombinant histones and nucleosome reconstitution\" >10804500</a ><br /> </div> </div> </div> <div id=\"prodAccordion\"> <div id=\"ProductDescription\" class=\"Block Panel\"> <h3 class=\"sub-title1\">Documents & Resources</h3> <div class=\"ProductDescriptionContainer product-droppdown__section-description\"> <div class=\"product-documents\"> <a href=\"/content/documents/tds/18-0005.pdf\" target=\"_blank\" class=\"product-documents__link\"> <svg version=\"1.1\" id=\"Layer_1\" xmlns=\"http://www.w3.org/2000/svg\" xmlns:xlink=\"http://www.w3.org/1999/xlink\" x=\"0px\" y=\"0px\" viewBox=\"0 0 228 240\" style=\"enable-background: new 0 0 228 240\" xml:space=\"preserve\" class=\"product-documents__icon\" alt=\"16-0030 Datasheet\"> <g> <path class=\"product-documents__svg-pdf\" d=\"M191.92,68.77l-47.69-47.69c-1.33-1.33-3.12-2.08-5.01-2.08H45.09C41.17,19,38,22.17,38,26.09v184.36 c0,3.92,3.17,7.09,7.09,7.09h141.82c3.92,0,7.09-3.17,7.09-7.09V73.8C194,71.92,193.25,70.1,191.92,68.77z M177.65,77.06h-41.7 v-41.7L177.65,77.06z M178.05,201.59H53.95V34.95h66.92v47.86c0,5.14,4.17,9.31,9.31,9.31h47.86V201.59z\" /> </g> <rect x=\"20\" y=\"112\" class=\"product-documents__svg-background\" width=\"146\" height=\"76\" /> <g> <path class=\"product-documents__svg-pdf\" d=\"M23.83,125.68h22.36c5.29,0,9.41,1.33,12.35,4c2.94,2.67,4.42,6.39,4.42,11.18c0,4.78-1.47,8.51-4.42,11.18 c-2.94,2.67-7.06,4-12.35,4H34.59v18.29H23.83V125.68z M44.81,147.9c5.38,0,8.07-2.32,8.07-6.97c0-2.39-0.67-4.16-2-5.31 c-1.33-1.15-3.36-1.73-6.07-1.73H34.59v14.01H44.81z\" /> <path class=\"product-documents__svg-pdf\" d=\"M69.92,125.68h18.91c5.29,0,9.84,0.97,13.66,2.9c3.82,1.93,6.74,4.72,8.76,8.35 c2.02,3.63,3.04,7.98,3.04,13.04c0,5.06-1,9.42-3,13.08c-2,3.66-4.91,6.45-8.73,8.38c-3.82,1.93-8.4,2.9-13.73,2.9H69.92V125.68z M88.07,165.63c10.35,0,15.52-5.22,15.52-15.66c0-10.4-5.17-15.59-15.52-15.59h-7.38v31.26H88.07z\" /> <path class=\"product-documents__svg-pdf\" d=\"M122.57,125.68h32.84v8.49h-22.22v11.18h20.84v8.49h-20.84v20.49h-10.63V125.68z\" /> </g> </svg> <span class=\"product-documents__info\">Technical Datasheet</span> </a> </div> </div> </div> </div> </div> </div>","tags":[],"warranty":"","price":{"without_tax":{"formatted":"$115.00","value":115,"currency":"USD"},"tax_label":"Tax"},"detail_messages":"","availability":"","page_title":"Nucleosome Assembly 601 Sequence DNA, Biotinylated for chromatin assembly and nucleosome reconstitution, useful for pull-down and binding assays","cart_url":"https://epicypher-c9.mybigcommerce.com/cart.php","max_purchase_quantity":0,"mpn":null,"upc":"50 µg","options":[],"related_products":[{"id":744,"sku":"16-0027","name":"Tailless Nucleosomes, Recombinant Human, Biotinylated","url":"https://epicypher-c9.mybigcommerce.com/products/nucleosomes/recombinant-nucleosomes-rnucs/tailless-nucleosomes-recombinant-human-biotinylated","availability":"","rating":0,"brand":{"name":null},"category":["Nucleosomes/Recombinant Nucleosomes (rNucs)"],"summary":" Tailless Nucleosomes, Human Recombinant, Biotinylated Description:","image":null,"images":[],"date_added":"18th Jan 2024","pre_order":false,"show_cart_action":true,"has_options":false,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":561,"name":"Pack Size","value":"50 μg"}],"num_reviews":0,"weight":{"formatted":"0.01 Ounces","value":0.01},"demo":false,"add_to_cart_url":"https://epicypher-c9.mybigcommerce.com/cart.php?action=add&product_id=744","price":{"without_tax":{"currency":"USD","formatted":"$575.00","value":575},"tax_label":"Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=744"},{"id":693,"sku":"16-0379","name":"Nucleosome, 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Pack Size: 50 μg
