SKU: 16-0016
{"num_reviews":0,"url":"https://epicypher-c9.mybigcommerce.com/products/nucleosomes/recombinant-nucleosomes-rnucs/mononucleosomes-h3-1-n32-recombinant-human-biotinylated","add_this":[{"service":"facebook","annotation":""},{"service":"twitter","annotation":""},{"service":"google","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""}],"gtin":null,"id":542,"can_purchase":true,"meta_description":"Mononucleosomes (H3.1 ΔN32), Recombinant Human Biotinylated for Enzyme Screening","category":["Nucleosomes/Recombinant Nucleosomes (rNucs)"],"AddThisServiceButtonMeta":"","main_image":null,"add_to_wishlist_url":"/wishlist.php?action=add&product_id=542","custom_fields":[{"id":"374","name":"Pack Size","value":"50 µg"}],"sku":"16-0016","description":"<div class=\"product-general-info\"> <ul class=\"product-general-info__list-left\"> <li class=\"product-general-info__list-item\"> <strong>Species: </strong>Human </li> <li class=\"product-general-info__list-item\"> <strong>Source: </strong><em>E. coli</em> & synthetic DNA </li> </ul> <ul class=\"product-general-info__list-right\"> <li class=\"product-general-info__list-item\"> <strong>Tag: </strong>Biotinylated </li> <li class=\"product-general-info__list-item\"> <strong>Molecular Weight: </strong>193,951.1 Da </li> </ul> </div> <div class=\"service_accordion product-droppdown\"> <div class=\"container\"> <div id=\"prodAccordion\"> <div id=\"ProductDescription\" class=\"Block Panel current\"> <h3 class=\"sub-title1\">Description</h3> <div class=\"ProductDescriptionContainer product-droppdown__section-description-specific\" > <p> Recombinant mononucleosomes (H3.1ΔN32) consist of 147 base pairs of DNA wrapped around an octamer core of histone proteins (two each of H2A, H2B, H3.1, and H4) to form a nucleosome, the basic repeating unit of chromatin. The 147 bp 601 sequence, identified by Lowary and Widom [1], has high affinity for histone octamers and is useful for nucleosome assembly. The amino acid sequence of H3.1 begins with glycine 33 (amino acids 1-32 are deleted) and the DNA in this nucleosome contains a 5’ biotin-TEG group. </p> </div> </div> </div> <div id=\"prodAccordion\"> <div id=\"ProductDescription\" class=\"Block Panel current\"> <h3 class=\"sub-title1\">Validation Data</h3> <div class=\"ProductDescriptionContainer product-droppdown__section-description-specific\" > <section class=\"image-picker\"> <div class=\"image-picker__left\"> <div class=\"image-picker__main-content_active image-picker__main-content\" > <div class=\"image-picker__header-content\"> <button class=\"image-picker__left-arrow\"> <svg class=\"image-picker__svg-left\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\" > <path d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" /> </svg> </button> <a href=\"/content/images/products/nucleosomes/16-0016-western-blot-data.jpeg\" target=\"_blank\" class=\"image-picker__main-image-link\" ><img alt=\"16-0016-western-blot-data\" src=\"/content/images/products/nucleosomes/16-0016-western-blot-data.jpeg\" class=\"image-picker__main-image\" /> <span class=\"image-picker__main-image-caption\" >(Click to enlarge)</span ></a > <button class=\"image-picker__right-arrow\"> <svg class=\"image-picker__svg-right\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\" > <path d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" /> </svg> </button> </div> <p> <span class=\"image-picker__span-content\" ><strong>Figure 1: Western blot data </strong><br /> Western analysis of H3.1ΔN32 nucleosome. <strong>Top Panel:</strong> WT H3.1 (Lane 1) and H3.1ΔN32-containing (Lane 2) nucleosomes were probed with an anti-H3 COOH-terminal antibody and analyzed via ECL readout. <strong>Bottom Panel:</strong> Detail from Coomassie stained gel showing histones from WT H3.1 (Lane 1) and H3.1ΔN32 nucleosomes (Lane 2). H3.1ΔN32 and H4 co-migrate. </span> </p> </div> <div class=\"image-picker__main-content\"> <div class=\"image-picker__header-content\"> <button class=\"image-picker__left-arrow\"> <svg class=\"image-picker__svg-left\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\" > <path d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" /> </svg> </button> <a href=\"/content/images/products/nucleosomes/16-0016-protein-gel-data.jpeg\" target=\"_blank\" class=\"image-picker__main-image-link\" ><img alt=\"16-0016-protein-gel-data\" src=\"/content/images/products/nucleosomes/16-0016-protein-gel-data.jpeg\" class=\"image-picker__main-image\" /> <span class=\"image-picker__main-image-caption\" >(Click to enlarge)</span ></a > <button class=\"image-picker__right-arrow\"> <svg class=\"image-picker__svg-right\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\" > <path d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" /> </svg> </button> </div> <p> <span class=\"image-picker__span-content\" ><strong>Figure 2: Protein gel data </strong><br /> Coomassie stained SDS-PAGE gel of proteins in H3.1ΔN32 nucleosome (1 μg) demonstrates the purity of histones in the preparation. Sizes of molecular weight markers and positions of the core histones (H2A, H2B, H3.1ΔN32 and H4) are indicated. H3.1ΔN32 and H4 co-migrate. </span> </p> </div> <div class=\"image-picker__main-content\"> <div class=\"image-picker__header-content\"> <button class=\"image-picker__left-arrow\"> <svg class=\"image-picker__svg-left\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\" > <path d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" /> </svg> </button> <a href=\"/content/images/products/nucleosomes/16-0016-dna-gel-data.jpeg\" target=\"_blank\" class=\"image-picker__main-image-link\" ><img alt=\"16-0016-dna-gel-data\" src=\"/content/images/products/nucleosomes/16-0016-dna-gel-data.jpeg\" class=\"image-picker__main-image\" /> <span class=\"image-picker__main-image-caption\" >(Click to enlarge)</span ></a > <button class=\"image-picker__right-arrow\"> <svg class=\"image-picker__svg-right\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\" > <path d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" /> </svg> </button> </div> <p> <span class=\"image-picker__span-content\" ><strong>Figure 3: DNA gel data </strong><br /> H3.1ΔN32 nucleosome resolved via native PAGE and stained with ethidium bromide to visualize DNA. <strong>Lane 1</strong>: Free DNA (EpiCypher <a href=\"https://www.epicypher.com/products/nucleosomes/nucleosome-assembly-601-sequence-dna-biotinylated\" >18-0005</a >; 100 ng). <strong>Lane 2:</strong> Intact H3.1ΔN32 nucleosomes (400 ng). </span> </p> </div> </div> <aside class=\"image-picker__right\"> <div class=\"image-picker__gallery\"> <img alt=\"16-0016-western-blot-data\" src=\"/content/images/products/nucleosomes/16-0016-western-blot-data.jpeg\" width=\"200\" class=\"image-picker__side-image image-picker__side-image_active\" role=\"button\" /> <img alt=\"16-0016-protein-gel-data\" src=\"/content/images/products/nucleosomes/16-0016-protein-gel-data.jpeg\" width=\"200\" class=\"image-picker__side-image image-picker__side-image_active\" role=\"button\" /> <img alt=\"16-0016-dna-gel-data\" src=\"/content/images/products/nucleosomes/16-0016-dna-gel-data.jpeg\" width=\"200\" class=\"image-picker__side-image image-picker__side-image_active\" role=\"button\" /> </div> </aside> </section> </div> </div> </div> <div id=\"prodAccordion\"> <div id=\"ProductDescription\" class=\"Block Panel\"> <h3 class=\"sub-title1\">Technical Information</h3> <div class=\"ProductDescriptionContainer product-droppdown__section-description\" > <div class=\"product-tech-info\"> <div class=\"product-tech-info__line-item\"> <div class=\"product-tech-info__line-item-left\"> <b>Storage</b> </div> <div class=\"product-tech-info__line-item-right\"> Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid freeze/thaws. </div> </div> <div class=\"product-tech-info__line-item\"> <div class=\"product-tech-info__line-item-left\"> <b>Formulation</b> </div> <div class=\"product-tech-info__line-item-right\"> 10 mM Tris-HCl pH 7.5, 1 mM EDTA, 25 mM NaCl, 2 mM DTT, 20% glycerol (27.4 µg protein, 50 µg DNA + protein) </div> </div> </div> </div> </div> </div> <div id=\"prodAccordion\"> <div id=\"ProductDescription\" class=\"Block Panel\"> <h3 class=\"sub-title1\">Application Notes</h3> <div class=\"ProductDescriptionContainer product-droppdown__section-description\" > <p> H3.1ΔN32 mononucleosome is highly purified and suitable for a variety of applications, including use as a substrate in enzyme assays, high-throughput screening and inhibitor testing, chromatin binding studies, protein-protein interaction assays, structural studies, and in effector protein binding experiments. The N-terminal deletion enables study of its role in chromatin biology. </p> </div> </div> </div> <div id=\"prodAccordion\"> <div id=\"ProductDescription\" class=\"Block Panel\"> <h3 class=\"sub-title1\">Gene & Protein Information</h3> <div class=\"ProductDescriptionContainer product-droppdown__section-description\" > a <div class=\"product-tech-info\"> <div class=\"product-tech-info__line-item\"> <div class=\"product-tech-info__line-item-left\"> <b>UniProt ID</b> </div> <div class=\"product-tech-info__line-item-right\"> <p style=\"margin-bottom: 0\"> H2A - P04908 (alt. names: H2A type 1-B/E, H2A.2, H2A/a, H2A/m) </p> <p style=\"margin-bottom: 0\"> H2B - O60814 (alt. names: H2B K, HIRA-interacting protein 1) </p> <p style=\"margin-bottom: 0\"> H3.1 - P68431 (alt. names: H3, H3/a, H3/b, H3/c, H3/d) </p> <p>H4 - P62805</p> </div> </div> </div> </div> </div> </div> <div id=\"prodAccordion\"> <div id=\"ProductDescription\" class=\"Block Panel\"> <h3 class=\"sub-title1\">References</h3> <div class=\"ProductDescriptionContainer product-droppdown__section-description\" > <strong>Background References:</strong> <br /> [1] Lowary & Widom <em>J. Mol. Biol.</em> (1998). PMID: <a href=\"https://pubmed.ncbi.nlm.nih.gov/9514715/\" title=\"New DNA sequence rules for high affinity binding to histone octamer and sequence-directed nucleosome positioning\" target=\"new\" > 9514715</a ><br /> </div> </div> </div> <div id=\"prodAccordion\"> <div id=\"ProductDescription\" class=\"Block Panel\"> <h3 class=\"sub-title1\">Documents & Resources</h3> <div class=\"ProductDescriptionContainer product-droppdown__section-description\" > <div class=\"product-documents\"> <a href=\"/content/documents/tds/16-0016.pdf\" target=\"_blank\" class=\"product-documents__link\" > <svg version=\"1.1\" id=\"Layer_1\" xmlns=\"http://www.w3.org/2000/svg\" xmlns:xlink=\"http://www.w3.org/1999/xlink\" x=\"0px\" y=\"0px\" viewBox=\"0 0 228 240\" style=\"enable-background: new 0 0 228 240\" xml:space=\"preserve\" class=\"product-documents__icon\" alt=\"16-0030 Datasheet\" > <g> <path class=\"product-documents__svg-pdf\" 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c2.02,3.63,3.04,7.98,3.04,13.04c0,5.06-1,9.42-3,13.08c-2,3.66-4.91,6.45-8.73,8.38c-3.82,1.93-8.4,2.9-13.73,2.9H69.92V125.68z M88.07,165.63c10.35,0,15.52-5.22,15.52-15.66c0-10.4-5.17-15.59-15.52-15.59h-7.38v31.26H88.07z\" /> <path class=\"product-documents__svg-pdf\" d=\"M122.57,125.68h32.84v8.49h-22.22v11.18h20.84v8.49h-20.84v20.49h-10.63V125.68z\" /> </g> </svg> <span class=\"product-documents__info\">Technical Datasheet</span> </a> </div> </div> </div> </div> </div> </div>","tags":[],"warranty":"","price":{"without_tax":{"formatted":"$475.00","value":475,"currency":"USD"},"tax_label":"Tax"},"detail_messages":"","availability":"","page_title":"Mononucleosomes (H3.1 ΔN32), Recombinant Human Biotinylated for Enzyme Screening","cart_url":"https://epicypher-c9.mybigcommerce.com/cart.php","max_purchase_quantity":0,"mpn":null,"upc":null,"options":[],"related_products":[{"id":543,"sku":"16-0017","name":"Mononucleosomes (H3.3 ΔN32), Recombinant Human 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Pack Size: 50 µg
