SKU: 15-0074
{"num_reviews":0,"url":"https://epicypher-c9.mybigcommerce.com/products/recombinant-proteins/hp1-recombinant-human-his-tagged","add_this":[{"service":"facebook","annotation":""},{"service":"twitter","annotation":""},{"service":"google","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""}],"gtin":null,"id":738,"can_purchase":true,"meta_description":"","category":["Recombinant Proteins/Histone Binding Domains","Recombinant Proteins/Histone Binding Domains/Chromodomain Proteins","Recombinant Proteins"],"AddThisServiceButtonMeta":"","main_image":null,"add_to_wishlist_url":"/wishlist.php?action=add&product_id=738","custom_fields":[{"id":"555","name":"Pack Size","value":"100 µg"}],"sku":"15-0074","description":"<div class=\"product-general-info\"> <ul class=\"product-general-info__list-left\"> <li class=\"product-general-info__list-item\"> <strong>Type: </strong>Chromodomain </li> <li class=\"product-general-info__list-item\"> <strong>Mol. Wgt.: </strong>24.4 kDa </li> </ul> <ul class=\"product-general-info__list-right\"> <li class=\"product-general-info__list-item\"> <strong>Expressed In : </strong><em>E. coli</em> </li> <li class=\"product-general-info__list-item\"> <strong>Epitope Tag: </strong>6xHis </li> </ul> </div> <div class=\"service_accordion product-droppdown\"> <div class=\"container\"> <div id=\"prodAccordion\"> <div id=\"ProductDescription\" class=\"Block Panel current\"> <h3 class=\"sub-title1\">Description</h3> <div class=\"ProductDescriptionContainer product-droppdown__section-description-specific\" > <p> Recombinant human HP1β (CBX1, M31, Heterochromatin protein 1 beta, accession P83916, amino acids 1-184) containing an N-terminal 6xHis tag, expressed in <em>E. coli</em>. HP1β is a component of constitutive (transcriptionally silent) heterochromatin, recruited through the interaction. </p> </div> </div> </div> <div id=\"prodAccordion\"> <div id=\"ProductDescription\" class=\"Block Panel current\"> <h3 class=\"sub-title1\">Validation Data</h3> <div class=\"ProductDescriptionContainer product-droppdown__section-description-specific\" > <section class=\"image-picker\"> <div class=\"image-picker__left\"> <div class=\"image-picker__main-content_active image-picker__main-content\" > <div class=\"image-picker__header-content\"> <button class=\"image-picker__left-arrow\"> <svg class=\"image-picker__svg-left\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\" > <path d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" /> </svg> </button> <a href=\"/content/images/products/proteins/15_0074_gel_web.jpg\" target=\"_blank\" class=\"image-picker__main-image-link\" ><img alt=\"15_0074_gel_web\" src=\"/content/images/products/proteins/15_0074_gel_web.jpg\" class=\"image-picker__main-image\" /> <span class=\"image-picker__main-image-caption\" >(Click to enlarge)</span ></a > <button class=\"image-picker__right-arrow\"> <svg class=\"image-picker__svg-right\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\" > <path d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" /> </svg> </button> </div> <p> <span class=\"image-picker__span-content\" ><strong>Figure 1: Protein gel data </strong><br /> HP1β, Recombinant Human (1 µg) was run on a PAGE gel and stained with Coomassie blue. The migration and molecular weight of the protein standards are indicated. </span> </p> </div> <div class=\"image-picker__main-content\"> <div class=\"image-picker__header-content\"> <button class=\"image-picker__left-arrow\"> <svg class=\"image-picker__svg-left\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\" > <path d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" /> </svg> </button> <a href=\"/content/images/products/proteins/15_0074_alpha.jpg\" target=\"_blank\" class=\"image-picker__main-image-link\" ><img alt=\"15_0074_alpha\" src=\"/content/images/products/proteins/15_0074_alpha.jpg\" class=\"image-picker__main-image\" /> <span class=\"image-picker__main-image-caption\" >(Click to enlarge)</span ></a > <button class=\"image-picker__right-arrow\"> <svg class=\"image-picker__svg-right\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\" > <path d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" /> </svg> </button> </div> <p> <span class=\"image-picker__span-content\" ><strong>Figure 2: AlphaScreen data</strong><br /> HP1β, Recombinant Human, His-Tagged used in nucleosome binding studies to detect interactions with specific post-translational modifications. The identities of the nucleosomes tested are as follows: rNuc unmodified (black), H3K9me3 (blue). </span> </p> </div> </div> <aside class=\"image-picker__right\"> <div class=\"image-picker__gallery\"> <img alt=\"15_0074_gel_web\" src=\"/content/images/products/proteins/15_0074_gel_web.jpg\" width=\"200\" class=\"image-picker__side-image image-picker__side-image_active\" role=\"button\" /> <img alt=\"15_0074_alpha\" src=\"/content/images/products/proteins/15_0074_alpha.jpg\" class=\"image-picker__side-image\" role=\"button\" /> </div> </aside> </section> </div> </div> </div> <div id=\"prodAccordion\"> <div id=\"ProductDescription\" class=\"Block Panel\"> <h3 class=\"sub-title1\">Technical Information</h3> <div class=\"ProductDescriptionContainer product-droppdown__section-description\" > <div class=\"product-tech-info\"> <div class=\"product-tech-info__line-item\"> <div class=\"product-tech-info__line-item-left\"> <b>Formulation</b> </div> <div class=\"product-tech-info__line-item-right\"> Recombinant 6xHis-tagged protein in 20 mM Tris-HCl pH 7.5, 100 mM NaCl, 1 mM DTT, 5% glycerol. </div> </div> <div class=\"product-tech-info__line-item\"> <div class=\"product-tech-info__line-item-left\"> <b>Storage and Stability</b> </div> <div class=\"product-tech-info__line-item-right\"> Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws. </div> </div> </div> </div> </div> </div> <div id=\"prodAccordion\"> <div id=\"ProductDescription\" class=\"Block Panel\"> <h3 class=\"sub-title1\">Application Notes</h3> <div class=\"ProductDescriptionContainer product-droppdown__section-description\" > <p> HP1β, Recombinant Human, His-Tagged is useful for protein binding and screening experiments examining methylated protein and peptide substrates. </p> </div> </div> </div> <div id=\"prodAccordion\"> <div id=\"ProductDescription\" class=\"Block Panel\"> <h3 class=\"sub-title1\">References</h3> <div class=\"ProductDescriptionContainer product-droppdown__section-description\" > <strong>Background References:</strong> <br /> [1] Li et al. <em>Cell</em> (2014). PMID: <a href=\"https://pubmed.ncbi.nlm.nih.gov/25417107/\" title=\"AF9 YEATS domain links histone acetylation to DOT1L-mediated H3K79 methylation\" target=\"new\" > 25417107</a ><br /> <br /> <strong>Product References:</strong> <iframe class=\"airtable-embed\" src=\"https://airtable.com/embed/shrvocttUukaQa7rG?backgroundColor=purple&viewControls=on\" frameborder=\"0\" onmousewheel=\"\" width=\"100%\" height=\"300\" style=\"background: transparent; border: 1px solid #ccc\" ></iframe> </div> </div> </div> <div id=\"prodAccordion\"> <div id=\"ProductDescription\" class=\"Block Panel\"> <h3 class=\"sub-title1\">Documents & Resources</h3> <div class=\"ProductDescriptionContainer product-droppdown__section-description\" > <div class=\"product-documents\"> <a href=\"/content/documents/tds/15-0074.pdf\" target=\"_blank\" class=\"product-documents__link\" > <svg version=\"1.1\" id=\"Layer_1\" xmlns=\"http://www.w3.org/2000/svg\" xmlns:xlink=\"http://www.w3.org/1999/xlink\" x=\"0px\" y=\"0px\" 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class=\"product-documents__svg-pdf\" d=\"M69.92,125.68h18.91c5.29,0,9.84,0.97,13.66,2.9c3.82,1.93,6.74,4.72,8.76,8.35 c2.02,3.63,3.04,7.98,3.04,13.04c0,5.06-1,9.42-3,13.08c-2,3.66-4.91,6.45-8.73,8.38c-3.82,1.93-8.4,2.9-13.73,2.9H69.92V125.68z M88.07,165.63c10.35,0,15.52-5.22,15.52-15.66c0-10.4-5.17-15.59-15.52-15.59h-7.38v31.26H88.07z\" /> <path class=\"product-documents__svg-pdf\" d=\"M122.57,125.68h32.84v8.49h-22.22v11.18h20.84v8.49h-20.84v20.49h-10.63V125.68z\" /> </g> </svg> <span class=\"product-documents__info\">Technical Datasheet</span> </a> </div> </div> </div> </div> </div> </div>","tags":[],"warranty":"","price":{"without_tax":{"formatted":"$325.00","value":325,"currency":"USD"},"tax_label":"Tax"},"detail_messages":"","availability":"","page_title":"","cart_url":"https://epicypher-c9.mybigcommerce.com/cart.php","max_purchase_quantity":0,"mpn":null,"upc":null,"options":[],"related_products":[{"id":277,"sku":"16-0009","name":"Mononucleosomes, Recombinant 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Pack Size: 100 µg
