SKU: 21-0013
{"num_reviews":0,"url":"https://epicypher-c9.mybigcommerce.com/products/recombinant-proteins/modifying-enzymes/5x-acf-remodeling-assay-buffer","add_this":[{"service":"facebook","annotation":""},{"service":"twitter","annotation":""},{"service":"google","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""}],"gtin":null,"id":644,"can_purchase":true,"meta_description":"5x ACF Remodeling Assay Buffer, ACF Remodeling Assay Buffer, Remodeling Assay Buffer,ACF Remodeling Enzyme Complex, ACF, Remodeling Enzyme Complex, chromatin remodeling complex, EpiDyne control, EpiDyne,","category":["Recombinant Proteins/Modifying Enzymes"],"AddThisServiceButtonMeta":"","main_image":null,"add_to_wishlist_url":"/wishlist.php?action=add&product_id=644","custom_fields":[{"id":"477","name":"Pack Size","value":"500 µL"}],"sku":"21-0013","description":"<table width=\"100%\" border=\"0\" cellspacing=\"2\" cellpadding=\"2\"> <tbody> <tr valign=\"top\"> <td> <table width=\"95%\" border=\"0\" cellspacing=\"1\" cellpadding=\"1\"> <tbody> <tr> <td> <table width=\"100%\" border=\"0\" cellspacing=\"0\" cellpadding=\"0\"> <tbody> <tr></tr> </tbody> </table> </td> </tr> <tr> <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b><span class=\"Apple-style-span\" style=\"font-family: Arial; \"><span class=\"Apple-style-span\" style=\"font-size: 12pt; \"><b>5x ACF Remodeling Assay Buffer<br> </b></span></span> Description:</b> 5x concentrated reaction buffer for use with ACF Remodeling Enzyme Complex (Catalog No. 15-1013). Optimized for use with recombinant Drosophila ACF Remodeling Enzyme Complex, assembled from Acf1-FLAG and ISWI produced in SF9 cells. ACF is an ATP-dependent chromatin remodeling complex that regulates nucleosome spacing.</span></span> </td> </tr> <tr> <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b><span class=\"Apple-style-span\" style=\"font-family: Arial; \"><span class=\"Apple-style-span\" style=\"font-size: 12pt; \"><b>ACF Remodeling Enzyme Complex</b></span></span> Formulation:</b> 5x ACF Remodeling Assay Buffer (100 mM Tris HCl, pH 7.5, 250 mM KCl, 15 mM MgCl2, 0.05% (w/v) BSA, 0.05% (v/v) Tween 20) is formulated for use with ACF Remodeling Enzyme Complex.<br /> <br /> </span></span> </td> </tr> <tr> <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b>Storage and Stability:</b> Stable for six months at 4°C from date of receipt.<br /> <br /> </span></span> </td> </tr> <tr> <td valign=\"top\"><p><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><b>Application Notes:</b> This produt is formulated to perform remodeling reactions using ACF Remodeling Enzyme Complex and EpiDyne®-FRET substrate in a reaction defined as 10 μl containing [5 nM ACF Complex/ 20 nM EpiDyne®-FRET/ 50 μM rATP] and remodels to completion in <20 minutes. For best results, store 5x ACF Remodeling Assay buffer at 4ºC and avoid freezing.</span></span></p> <p><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"> For further information, please contact techsupport@epicypher.com or refer to the EpiDyne®- FRET Technote (TN#106; available at www.epicypher.com/technotes).</span></span><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><br /> </span></span></p> </td> </tr> <tr> <td valign=\"top\"><p><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\"><br /> </span></span><br /> </p> </td> </tr> <tr> <td valign=\"top\"><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 12pt; \" class=\"Apple-style-span\">View technical datasheet for this product. <a href=\"https://www.epicypher.com/content/documents/tds/21-0013.pdf\" target=\"_blank\"><img src=\"https://cdn7.bigcommerce.com/s-y9o92/content/documents/tds/icon.png\" alt=\"21-0013 Datasheet\" width=\"30\" height=\"40\" /></a></span></span> </td> </tr> </tbody> </table> </td> <td> <table width=\"100%\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\"> <tbody> <tr> <td align=\"center\"><a href=\"https://www.epicypher.com/content/images/products/proteins/15_1013_ATP_dep_chromatin_remodeling.jpg\" target=\"_new\"> <img src=\"https://cdn7.bigcommerce.com/s-y9o92/content/images/products/proteins/15_1013_ATP_dep_chromatin_remodeling.jpg\" alt=\"15-1013 ATP-dependent Chromatin Remodeling \" width=\"250\" height=\"183\" /></a> </td> </tr> <tr> <td align=\"left\" valign=\"top\"> <p><span style=\"font-family: Arial; \" class=\"Apple-style-span\"><span style=\"font-size: 10pt; \" class=\"Apple-style-span\"><strong>ATP-dependent Chromatin Remodeling Assay:</strong> EpiDyne®- FRET Chromatin Remodeling Substrate (20 nM; Catalog No. 16-4201) incubated with ACF Remodeling Enzyme Complex (Catalog No. 15-1013) using ACF Remodeling Assay Buffer with or without ATP (50 μM). Curves denote FRET efficiency/chromatin remodeling.<br /> <strong>(Click image to enlarge) </strong></span></span> </p></td> </tr> </tbody> </table> <table width=\"100%\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\"> <tbody> <tr> <td align=\"center\"></td> </tr> <tr> <td align=\"left\" valign=\"top\"></td> </tr> </tbody> </table> </td> </tr> </tbody> </table> </body> </html>","tags":[],"warranty":"","price":{"without_tax":{"formatted":"$55.00","value":55,"currency":"USD"},"tax_label":"Tax"},"detail_messages":"","availability":"","page_title":"5x ACF Remodeling Assay Buffer","cart_url":"https://epicypher-c9.mybigcommerce.com/cart.php","max_purchase_quantity":0,"mpn":null,"upc":null,"options":[],"related_products":[{"id":572,"sku":"15-1013","name":"ACF Chromatin Remodeling Enzyme 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Pack Size: 500 µL
