SKU: 15-1015
{"num_reviews":0,"url":"https://epicypher-c9.mybigcommerce.com/products/nucleosomes/epidyne-chromatin-remodeling-substrates/epidyne-modifying-enzymes/smarca2-chromatin-remodeling-enzyme-human-brm","add_this":[{"service":"facebook","annotation":""},{"service":"twitter","annotation":""},{"service":"google","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""}],"gtin":null,"id":671,"can_purchase":true,"meta_description":"Enzymatically active full-length SMARCA2 (human BRM) for use in HTS drug discovery assays and chromatin binding experiments.","category":["Recombinant Proteins/Modifying Enzymes","Nucleosomes/EpiDyne® Chromatin Remodeling Substrates/EpiDyne<sup>®</sup> Modifying Enzymes","Recombinant Proteins/EpiDyne<sup>®</sup> Chromatin Remodeling Enzymes"],"AddThisServiceButtonMeta":"","main_image":null,"add_to_wishlist_url":"/wishlist.php?action=add&product_id=671","custom_fields":[{"id":"493","name":"Pack Size","value":"100 Reactions"},{"id":"494","name":"Internal Comment","value":"extra in top right of Psylocke"},{"id":"495","name":"Internal Comment","value":"bulk in LG"}],"sku":"15-1015","description":"<div class=\"product-general-info\"> <ul class=\"product-general-info__list-left\"> <li class=\"product-general-info__list-item\"> <strong>Species: </strong>Human </li> <li class=\"product-general-info__list-item\"> <strong>Source: </strong>SF9 cells </li> </ul> <ul class=\"product-general-info__list-right\"> <li class=\"product-general-info__list-item\"> <strong>Epitope Tag: </strong>6His-FLAG </li> <li class=\"product-general-info__list-item\"> <strong>Mol Wgt: </strong>181 kDa </li> </ul> </div> <div class=\"service_accordion product-droppdown\"> <div class=\"container\"> <div id=\"prodAccordion\"> <div id=\"ProductDescription\" class=\"Block Panel current\"> <h3 class=\"sub-title1\">Description</h3> <div class=\"ProductDescriptionContainer product-droppdown__section-description-specific\" style=\"display: block\"> <p> Full length recombinant human SMARCA2 Remodeling Enzyme. SMARCA2 is a core ATPase subunit of the ATP-dependent chromatin remodeling complex SWI/SNF that mobilizes nucleosomes and plays key roles in gene transcription regulation, development, genome replication and repair [1,2]. Aberrant activity of SWI/SNF subunits is linked with more than 20% of human cancers and multiple developmental disorders, making SMARCA2 an emerging candidate therapeutic target [3,4]. SMARCA2 can be used in conjunction with EpiDyne<sup>®</sup>-FRET Remodeling Assay Substrate (EpiCypher <a href=\"/products/nucleosomes/epidyne-fret-nucleosome-remodeling-assay-substrate\" >16-4201</a >) in HTS-compatible drug discovery studies. </p> <p style=\" background-color: #4698cb; color: #fff; padding: 1.3rem; text-align: center; border-radius: 12px; \"> <a target=\"_blank\" style=\"color: #fff\" href=\"/resources/protocols/epidyne-guide\" >Want to learn more? Check out our EpiDyne® Guide >> </a> </p> </div> </div> </div> <div id=\"prodAccordion\"> <div id=\"ProductDescription\" class=\"Block Panel current\"> <h3 class=\"sub-title1\">Validation Data</h3> <div class=\"ProductDescriptionContainer product-droppdown__section-description-specific\" style=\"display: block\"> <section class=\"image-picker\"> <div class=\"image-picker__left\"> <div class=\"image-picker__main-content_active image-picker__main-content\"> <div class=\"image-picker__header-content\"> <button class=\"image-picker__left-arrow\"> <svg class=\"image-picker__svg-left\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\"> <path d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" /> </svg> </button> <a href=\"/content/images/products/proteins/15-1015-chromatin-remodeling-assay.png\" target=\"blank\" class=\"image-picker__main-image-link\" ><img loading=\"lazy\" alt=\"15-1015-chromatin-remodeling-assay\" src=\"/content/images/products/proteins/15-1015-chromatin-remodeling-assay.png\" class=\"image-picker__main-image\" /> <span class=\"image-picker__main-image-caption\" >(Click to enlarge)</span ></a > <button class=\"image-picker__right-arrow\"> <svg class=\"image-picker__svg-right\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\"> <path d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" /> </svg> </button> </div> <p> <span class=\"image-picker__span-content\" ><strong >Figure 1: ATP-dependent chromatin remodeling assay </strong ><br /> EpiDyne<sup>®</sup>-FRET Chromatin Remodeling Substrate (EpiCypher <a href=\"/products/nucleosomes/epidyne-fret-nucleosome-remodeling-assay-substrate\" >16-4201</a >; 20 nM) incubated with SMARCA2 Remodeling Enzyme (concentrations indicated) in 1x SMARCA Remodeling Assay Buffer. Curves denote FRET efficiency/chromatin remodeling. </span> </p> </div> <div class=\"image-picker__main-content\"> <div class=\"image-picker__header-content\"> <button class=\"image-picker__left-arrow\"> <svg class=\"image-picker__svg-left\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\"> <path d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" /> </svg> </button> <a href=\"/content/images/products/proteins/15-1015-protein-gel-data.jpeg\" target=\"blank\" class=\"image-picker__main-image-link\" ><img loading=\"lazy\" alt=\"15-1015-protein-gel-data\" src=\"/content/images/products/proteins/15-1015-protein-gel-data.jpeg\" class=\"image-picker__main-image\" /> <span class=\"image-picker__main-image-caption\" >(Click to enlarge)</span ></a > <button class=\"image-picker__right-arrow\"> <svg class=\"image-picker__svg-right\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\"> <path d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" /> </svg> </button> </div> <p> <span class=\"image-picker__span-content\" ><strong>Figure 2: Protein gel data </strong><br /> SMARCA2 Remodeling Enzyme (1 µg) was run on an SDS-PAGE gel and stained with Coomassie blue. The migration and molecular weight of the protein standards are indicated. </span> </p> </div> </div> <aside class=\"image-picker__right\"> <div class=\"image-picker__gallery\"> <img loading=\"lazy\" alt=\"15-1015-chromatin-remodeling-assay\" src=\"/content/images/products/proteins/15-1015-chromatin-remodeling-assay.png\" width=\"200\" class=\"image-picker__side-image image-picker__side-image_active\" role=\"button\" /> <img loading=\"lazy\" alt=\"15-1015-protein-gel-data\" src=\"/content/images/products/proteins/15-1015-protein-gel-data.jpeg\" class=\"image-picker__side-image\" role=\"button\" /> </div> </aside> </section> </div> </div> </div> <div id=\"prodAccordion\"> <div id=\"ProductDescription\" class=\"Block Panel\"> <h3 class=\"sub-title1\">Technical Information</h3> <div class=\"ProductDescriptionContainer product-droppdown__section-description\"> <div class=\"product-tech-info\"> <div class=\"product-tech-info__line-item\"> <div class=\"product-tech-info__line-item-left\"> <b>Formulation:</b> </div> <div class=\"product-tech-info__line-item-right\"> 25 mM HEPES pH 7.6, 0.1 mM EDTA, 10% glycerol, 350 mM NaCl, 1 mM DTT, 0.4 mM PMSF, 1 mM benzamidine, 0.02% NP-40, 0.4 mg/mL insulin. </div> </div> <div class=\"product-tech-info__line-item\"> <div class=\"product-tech-info__line-item-left\"> <b>Storage and Stability:</b> </div> <div class=\"product-tech-info__line-item-right\"> Stable for six months -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws. </div> </div> </div> </div> </div> </div> <div id=\"prodAccordion\"> <div id=\"ProductDescription\" class=\"Block Panel\"> <h3 class=\"sub-title1\">Application Notes</h3> <div class=\"ProductDescriptionContainer product-droppdown__section-description\"> <p> This product is sufficient to perform 100 remodeling reactions using EpiDyne<sup>®</sup>-FRET substrate (EpiCypher <a href=\"/products/nucleosomes/epidyne-fret-nucleosome-remodeling-assay-substrate\" >16-4201</a >). A single reaction is defined as 10 µL containing a lot-specific concentration of SMARCA2 (see Technical Datasheet), 20 nM EpiDyne<sup>®</sup>-FRET, & 1 mM rATP with remodeling to completion in <30 minutes. Remodeling readout can be observed in the change of Cy3/Cy5 ratio over time in a black 384-well assay plate on a compatible fluorescent plate reader. 5x SMARCA Remodeling Assay Buffer is included (EpiCypher <a href=\"/products/recombinant-proteins/modifying-enzymes/5x-smarca-remodeling-assay-buffer\" >21-0014</a >). For more information, see the <a href=\"/resources/technical-notes/\" >EpiDyne<sup>®</sup>-FRET Technote</a > or contact <a href=\"mailto:techsupport@epicypher.com\" >techsupport@epicypher.com</a >. </p> </div> </div> </div> <div id=\"prodAccordion\"> <div id=\"ProductDescription\" class=\"Block Panel\"> <h3 class=\"sub-title1\">Gene & Protein Information</h3> <div class=\"ProductDescriptionContainer product-droppdown__section-description\"> <div class=\"product-tech-info\"> <div class=\"product-tech-info__line-item\"> <div class=\"product-tech-info__line-item-left\"> <b>UniProt ID</b> </div> <div class=\"product-tech-info__line-item-right\">P51531-2</div> </div> <div class=\"product-tech-info__line-item\"> <div class=\"product-tech-info__line-item-left\"> <b>Gene Name</b> </div> <div class=\"product-tech-info__line-item-right\">SMARCA2</div> </div> <div class=\"product-tech-info__line-item\"> <div class=\"product-tech-info__line-item-left\"> <b>Protein Name</b> </div> <div class=\"product-tech-info__line-item-right\"> Probable global transcription activator SNF2L2 </div> </div> <div class=\"product-tech-info__line-item\"> <div class=\"product-tech-info__line-item-left\"> <b>Amino Acids</b> </div> <div class=\"product-tech-info__line-item-right\"> 1-1572 with a C-terminal 6His-FLAG </div> </div> <div class=\"product-tech-info__line-item\"> <div class=\"product-tech-info__line-item-left\"> <b>Alternate Names</b> </div> <div class=\"product-tech-info__line-item-right\"> BAF190B, BRM, SNF2A, SNF2L2 </div> </div> </div> </div> </div> </div> <div id=\"prodAccordion\"> <div id=\"ProductDescription\" class=\"Block Panel\"> <h3 class=\"sub-title1\">References</h3> <div class=\"ProductDescriptionContainer product-droppdown__section-description\"> <strong>Background References:</strong> <br /> [1] Clapier & Carins <em>Annu. Rev. Biochem.</em> (2009). PMID: <a href=\"https://pubmed.ncbi.nlm.nih.gov/19355820/\" title=\"The biology of chromatin remodeling complexes\" target=\"new\"> 19355820</a ><br /> [2] Sundaramoorthy & Owen-Hughes <em>F1000Research</em> (2020). PMID: <a href=\"https://pubmed.ncbi.nlm.nih.gov/32864100/\" title=\"Chromatin remodelling comes into focus\" target=\"new\"> 32864100</a ><br /> [3] Kadoch & Crabtree <em>Sci. Adv.</em> (2015). PMID: <a href=\"https://pubmed.ncbi.nlm.nih.gov/26601204/\" title=\"Mammalian SWI/SNF chromatin remodeling complexes and cancer: Mechanistic insights gained from human genomics\" target=\"new\"> 26601204</a ><br /> [4] Cenik & Shilatifard <em>Nat. Rev</em> (2021). PMID: <a href=\"https://pubmed.ncbi.nlm.nih.gov/32958894/\" title=\"COMPASS and SWI/SNF complexes in development and disease\" target=\"new\"> 32958894</a ><br /> </div> </div> </div> <div id=\"prodAccordion\"> <div id=\"ProductDescription\" class=\"Block Panel\"> <h3 class=\"sub-title1\">Documents & Resources</h3> <div class=\"ProductDescriptionContainer product-droppdown__section-description\"> <div class=\"product-documents\"> <a href=\"/content/documents/tds/15-1015.pdf\" target=\"blank\" class=\"product-documents__link\"> <svg version=\"1.1\" id=\"Layer_1\" xmlns=\"http://www.w3.org/2000/svg\" xmlns:xlink=\"http://www.w3.org/1999/xlink\" x=\"0px\" y=\"0px\" viewBox=\"0 0 228 240\" style=\"enable-background: new 0 0 228 240\" xml:space=\"preserve\" class=\"product-documents__icon\"> <g> <path class=\"product-documents__svg-pdf\" d=\"M191.92,68.77l-47.69-47.69c-1.33-1.33-3.12-2.08-5.01-2.08H45.09C41.17,19,38,22.17,38,26.09v184.36 c0,3.92,3.17,7.09,7.09,7.09h141.82c3.92,0,7.09-3.17,7.09-7.09V73.8C194,71.92,193.25,70.1,191.92,68.77z M177.65,77.06h-41.7 v-41.7L177.65,77.06z M178.05,201.59H53.95V34.95h66.92v47.86c0,5.14,4.17,9.31,9.31,9.31h47.86V201.59z\" /> </g> <rect x=\"20\" y=\"112\" class=\"product-documents__svg-background\" width=\"146\" height=\"76\" /> <g> <path class=\"product-documents__svg-pdf\" d=\"M23.83,125.68h22.36c5.29,0,9.41,1.33,12.35,4c2.94,2.67,4.42,6.39,4.42,11.18c0,4.78-1.47,8.51-4.42,11.18 c-2.94,2.67-7.06,4-12.35,4H34.59v18.29H23.83V125.68z M44.81,147.9c5.38,0,8.07-2.32,8.07-6.97c0-2.39-0.67-4.16-2-5.31 c-1.33-1.15-3.36-1.73-6.07-1.73H34.59v14.01H44.81z\" /> <path class=\"product-documents__svg-pdf\" d=\"M69.92,125.68h18.91c5.29,0,9.84,0.97,13.66,2.9c3.82,1.93,6.74,4.72,8.76,8.35 c2.02,3.63,3.04,7.98,3.04,13.04c0,5.06-1,9.42-3,13.08c-2,3.66-4.91,6.45-8.73,8.38c-3.82,1.93-8.4,2.9-13.73,2.9H69.92V125.68z M88.07,165.63c10.35,0,15.52-5.22,15.52-15.66c0-10.4-5.17-15.59-15.52-15.59h-7.38v31.26H88.07z\" /> <path class=\"product-documents__svg-pdf\" d=\"M122.57,125.68h32.84v8.49h-22.22v11.18h20.84v8.49h-20.84v20.49h-10.63V125.68z\" /> </g> </svg> <span class=\"product-documents__info\">Technical Datasheet</span> </a> </div> </div> </div> </div> </div> </div>","tags":[],"warranty":"","price":{"without_tax":{"formatted":"$575.00","value":575,"currency":"USD"},"tax_label":"Tax"},"detail_messages":"","availability":"","page_title":"SMARCA2 Chromatin Remodeling Enzyme (Human BRM)","cart_url":"https://epicypher-c9.mybigcommerce.com/cart.php","max_purchase_quantity":0,"mpn":null,"upc":null,"options":[],"related_products":[{"id":672,"sku":"15-1014","name":"SMARCA4 Chromatin Remodeling Enzyme (Human 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7"}],"num_reviews":0,"weight":{"formatted":"0.01 Ounces","value":0.01},"demo":false,"add_to_cart_url":"https://epicypher-c9.mybigcommerce.com/cart.php?action=add&product_id=672","price":{"without_tax":{"currency":"USD","formatted":"$575.00","value":575},"tax_label":"Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=672"},{"id":572,"sku":"15-1013","name":"ACF Chromatin Remodeling Enzyme Complex","url":"https://epicypher-c9.mybigcommerce.com/products/nucleosomes/epidyne-chromatin-remodeling-substrates/epidyne-modifying-enzymes/acf-chromatin-remodeling-enzyme-complex","availability":"","rating":0,"brand":{"name":null},"category":["Recombinant Proteins/Modifying Enzymes","Nucleosomes/EpiDyne® Chromatin Remodeling Substrates/EpiDyne<sup>®</sup> Modifying Enzymes","Recombinant Proteins/EpiDyne<sup>®</sup> Chromatin Remodeling Enzymes"],"summary":" ","image":null,"images":[],"date_added":"18th Jan 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Pack Size: 100 Reactions
