SKU: 16-4115
{"num_reviews":0,"url":"https://epicypher-c9.mybigcommerce.com/products/nucleosomes/epidyne-nucleosome-remodeling-assay-substrate-st601-gatc1-2-50-n-66-biotinylated","add_this":[{"service":"facebook","annotation":""},{"service":"twitter","annotation":""},{"service":"google","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""}],"gtin":null,"id":741,"can_purchase":true,"meta_description":"","category":["Nucleosomes","Nucleosomes/EpiDyne® Chromatin Remodeling Substrates"],"AddThisServiceButtonMeta":"","main_image":null,"add_to_wishlist_url":"/wishlist.php?action=add&product_id=741","custom_fields":[{"id":"557","name":"Pack Size","value":"50 μg"},{"id":"558","name":"Internal Comment","value":"Bulk in LG"}],"sku":"16-4115","description":"<div> <table style=\"width: 100%;\" border=\"0\" cellspacing=\"2\" cellpadding=\"2\"> <tbody> <tr valign=\"top\"> <td> <table style=\"width: 95%;\" border=\"0\" cellspacing=\"1\" cellpadding=\"1\"> <tbody> <tr> <td valign=\"top\"><strong style=\"font-family: Arial; font-size: 16px;\">EpiDyne Nucleosome Remodeling Assay Substrate ST601-GATC1,2, 50-N-66, Biotinylated Description: </strong><span class=\"Apple-style-span\" style=\"font-family: Arial; font-size: 12pt;\">Mononucleosomes assembled from recombinant human histones expressed in <em>E. coli</em> (two each of histones H2A, H2B, H3 and H4. Accession numbers: H2A-P04908; H2B-O60814; H3.1-P68431; H4-P62805) wrapped by provided 263 base pair DNA sequence that includes the Widom 601 sequence with two added GATC sequences and a 5' biotin-TEG group. </strong> </span><br /> <br /> </p></td> </tr> <tr> <td valign=\"top\"><strong style=\"font-family: Arial; font-size: 16px;\">EpiDyne Nucleosome Remodeling Assay Substrate ST601-GATC1,2, 50-N-66, Biotinylated Formulation: </strong><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><span class=\"Apple-style-span\" style=\"font-size: 12pt;\">Purified recombinant mononucleosomes in 10mM Tris-HCl pH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM DTT, 20% glycerol. MW = 271,083.4 Da.<br /> <br /> </span></span></td> </tr> <tr> <td valign=\"top\"><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><span class=\"Apple-style-span\" style=\"font-size: 12pt;\"><strong style=\"font-family: Arial; font-size: 16px;\">EpiDyne Nucleosome Remodeling Assay Substrate ST601-GATC1,2, 50-N-66, Biotinylated Storage and Stability:</strong> Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws. <br /> <br /> </span></span></td> </tr> <tr> <td valign=\"top\"><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><span class=\"Apple-style-span\" style=\"font-size: 12pt;\"><strong style=\"font-family: Arial; font-size: 16px;\">EpiDyne Nucleosome Remodeling Assay Substrate ST601-GATC1,2, 50-N-66 Biotinylated Application Notes:</strong> This product is a template for nucleosome remodeling assays using the restriction enzyme DpnII to determine accessibility of GATCs which are masked in its native configuration (prior to remodeling). <br /> </span></span></td> </tr> <tr> <td valign=\"top\"><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><strong style=\"font-size: 12pt;\">References:</strong><br /> <br /> <br /> </span></td> </tr> <tr> <td valign=\"top\"> <p><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><span class=\"Apple-style-span\" style=\"font-size: 12pt;\">View technical datasheet for this product. <a href=\"https://www.epicypher.com/content/documents/tds/16-4115.pdf\" target=\"_blank\"> <img src=\"https://cdn7.bigcommerce.com/s-y9o92/content/documents/tds/icon.png\" alt=\"16-4115 Datasheet\" width=\"30\" height=\"40\" /></a></span></span></p> </td> </tr> </tbody> </table> </td> <td width=\"350px\"> <table style=\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\"> <tbody> <tr valign=\"top\"> <td align=\"center\" valign=\"top\"> <a href=\"https://www.epicypher.com/content/images/products/nucleosomes/16-4115_DNA_gel_tds.jpg\" target=\"_new\"> <img src=\"https://cdn7.bigcommerce.com/s-y9o92/content/images/products/nucleosomes/16-4115_DNA_gel_tds.jpg\" alt=\"16-4115 Protein Gel\" width=\"150\"/></a></td> </tr> <tr> <td align=\"left\" valign=\"top\"><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><span class=\"Apple-style-span\" style=\"font-size: 10pt;\"><strong>DNA Gel Data:</strong> Free DNA (DNA, 200 ng) and EpiDyne Nucleosome Remodeling Assay Substrate ST601-GATC1,2, 50-N-66, Biotinylated (Nuc, 400 ng) resolved via native PAGE and stained with ethidium bromide to visualize DNA. <br /> <strong>(Click image to enlarge)<strong style=\"font-family: Arial; font-size: 10pt;\"> </strong></span></span></td> </tr> <tr> <td align=\"center\"><a href=\"https://www.epicypher.com/content/images/products/nucleosomes/16-4115_protein_gel_tds.jpg\" target=\"_new\"> <img src=\"https://cdn7.bigcommerce.com/s-y9o92/content/images/products/nucleosomes/16-4115_protein_gel_tds.jpg\" alt=\"16-4115 DNA Gel\" width=\"200\" /></a></td> </tr> <tr> <td align=\"left\" valign=\"top\"><strong style=\"font-family: Arial; font-size: 10pt;\">Protein Gel Data:</strong><span style=\"font-family: Arial; font-size: 10pt;\"> Coomassie stained PAGE gel of proteins in <span class=\"Apple-style-span\" style=\"font-size: 10pt;\">Histone Proteins in EpiDyne Nucleosome Remodeling Assay Substrate ST601-GATC1,2, 50-N-66 Biotinylated resolved via SDS-PAGE and coomassie staining </span> (2 µg) . Sizes of molecular weight markers and positions of the core histones (H2A, H2B, H3 and H4) are indicated. <br /> <strong>(Click image to enlarge) </span></td> </tr> <tr> <td align=\"center\"><a href=\"https://www.epicypher.com/content/images/products/nucleosomes/16-4115_remodel_tds.jpg\" target=\"_new\"> <img src=\"https://cdn7.bigcommerce.com/s-y9o92/content/images/products/nucleosomes/16-4115_remodel_tds.jpg\" alt=\"16-4115 Western Blot\" width=\"250\" /></a></td> </tr> <tr> <td align=\"left\" valign=\"top\"><span style=\"font-size:10pt;\"><span style=\"font-family:arial,helvetica,sans-serif;\"><strong style=\"font-family: Arial; font-size: 10pt;\">Nucleosome Remodeling Data:</strong> ACF/ATP-dependent nucleosome remodeling reaction in the presence of DpnII restriction enzyme. <span class=\"Apple-style-span\" style=\"font-size: 10pt;\">EpiDyne Nucleosome Remodeling Assay Substrate ST601-GATC1,2, 50-N-66 Biotinylated</span> nucleosomes incubated with (10 nM) or without ACF for up to 9 minutes in the presence of 2 mM ATP (or No ATP, as indicated) and 50U of DpnII. Samples were quenched at specified intervals and resolved via native PAGE. <br /> <strong>(Click image to enlarge)</span></span></td> </tr> </tbody> </table> </td> </tr> </tbody> </table> </div>","tags":[],"warranty":"","price":{"without_tax":{"formatted":"$565.00","value":565,"currency":"USD"},"tax_label":"Tax"},"detail_messages":"","availability":"","page_title":"","cart_url":"https://epicypher-c9.mybigcommerce.com/cart.php","max_purchase_quantity":0,"mpn":null,"upc":null,"options":[],"related_products":[{"id":677,"sku":"16-4112","name":"EpiDyne® Nucleosome Remodeling Assay Substrate ST601-GATC1,2, Biotinylated","url":"https://epicypher-c9.mybigcommerce.com/products/nucleosomes/epidyne-chromatin-remodeling-substrates/epidyne-nucleosome-remodeling-assay-substrate-st601-gatc1-2-biotinylated","availability":"","rating":0,"brand":{"name":null},"category":["Nucleosomes/EpiDyne® Chromatin Remodeling Substrates","Discontinued Products"],"summary":" EpiDyne Nucleosome Remodeling Assay 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Pack Size: 50 μg
