SKU: 16-8012
{"num_reviews":0,"url":"https://epicypher-c9.mybigcommerce.com/products/nucleosomes/histone-octamer-h3-3-human-recombinant","add_this":[{"service":"facebook","annotation":""},{"service":"twitter","annotation":""},{"service":"google","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""}],"gtin":null,"id":642,"can_purchase":true,"meta_description":"Histone Octamer (H3.3), Human Recombinant for Enzyme Activity Screening","category":["Nucleosomes","Nucleosomes/Nucleosome Components and Subunits"],"AddThisServiceButtonMeta":"","main_image":null,"add_to_wishlist_url":"/wishlist.php?action=add&product_id=642","custom_fields":[{"id":"476","name":"Pack Size","value":"50 μg"}],"sku":"16-8012","description":"<body> <div> <table style=\"width: 100%;\" border=\"0\" cellspacing=\"2\" cellpadding=\"2\"> <tbody> <tr valign=\"top\"> <td> <table style=\"width: 95%;\" border=\"0\" cellspacing=\"1\" cellpadding=\"1\"> <tbody> <tr> <td valign=\"top\"><strong style=\"font-family: Arial; font-size: 16px;\">Histone Octamer (H3.3), Human Recombinantt </strong><strong style=\"font-family: Arial; font-size: 12pt;\">Description: </strong><span class=\"Apple-style-span\" style=\"font-family: Arial; font-size: 12pt;\">Human histone octamers assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3.3 and H4; accession numbers: H2A-P04908; H2B-O60814; H3.3-P84243; H4-P62805). The histone octamer is the protein component of the nucleosome, the basic subunit of chromatin. A nucleosome consists of a histone octamer wrapped with 147 base pairs of DNA (note that there is only histone octamer in this product).</span><br /> <br /> </p></td> </tr> <tr> <td valign=\"top\"><strong style=\"font-family: Arial; font-size: 16px;\">Histone Octamer (H3.3), Human Recombinantt Formulation: </strong><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><span class=\"Apple-style-span\" style=\"font-size: 12pt;\">Gel filtration-purified recombinant human histone octamers ( mg/ml) in μl of 10 mM Tris-HCl pH 7.5, 1 mM EDTA, 2 M NaCl, 2 mM DTT, 20% glycerol. MW: 108,392.2 Da. Molarity: μM.<br /> <br /> </span></span></td> </tr> <tr> <td valign=\"top\"><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><span class=\"Apple-style-span\" style=\"font-size: 12pt;\"><strong style=\"font-family: Arial; font-size: 16px;\">Histone Octamer (H3.3), Human Recombinantt </strong><strong>Storage and Stability:</strong> Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws. <br /> <br /> </span></span></td> </tr> <tr> <td valign=\"top\"><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><span class=\"Apple-style-span\" style=\"font-size: 12pt;\"><strong style=\"font-family: Arial; font-size: 16px;\">Histone Octamer (H3.3), Human Recombinantt </strong><strong>Application Notes:</strong> Human recombinant histone octamers can be used for chromatin reconstitution experiments, or as substrates for histone modifying enzymes. See product page on web for links to protocols for chromatin reconstitution. Histone octamers should not be stored under conditions where the salt concentration is lower than 800 mM. Please note that the concentration may vary compared to previous lots.</span></span></td> </tr> <tr> <td valign=\"top\"><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><strong style=\"font-size: 12pt;\">References:</strong><br /> <span style=\"font-size: 10pt;\">Lowary PT and J Widom (1998). <em>J Mol Bio</em>l 276: 19-42. <a href=\"http://www.ncbi.nlm.nih.gov/pubmed/9514715\" target=\"_new\">Link</a></span><br /> <span style=\"font-size: 10pt;\">Luger K et al (1999). <em>Methods Mol Biol</em> 119: 1-16. <a href=\"http://www.ncbi.nlm.nih.gov/pubmed/10804500\" target=\"_new\">Link</a></span><br /> <br /> </span></td> </tr> <tr> <td valign=\"top\"> <p><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><span class=\"Apple-style-span\" style=\"font-size: 12pt;\">View technical datasheet for this product. <a href=\"#\" target=\"_blank\"> <img src=\"https://cdn7.bigcommerce.com/s-y9o92/content/documents/tds/icon.png\" alt=\"16-8012 Datasheet\" width=\"30\" height=\"40\" /></a></span></span></p> </td> </tr> </tbody> </table> </td> <td> <table style=\"width: 100%;\" border=\"0\" cellspacing=\"3\" cellpadding=\"3\"> <tbody> <tr> <td align=\"center\"><a href=\"#\" target=\"_new\"> <img src=\"#\" alt=\"16-8012 DNA Gel\" width=\"281\" height=\"300\" /></a></td> </tr> <tr> <td align=\"left\" valign=\"top\"><strong style=\"font-family: Arial; font-size: 10pt;\">DNA Gel Data:</strong><span style=\"font-family: Arial; font-size: 10pt;\"> Histone Octamer (H3.3), Human Recombinantt run on an agarose gel and stained with ethidium bromide to visualize DNA. </span><strong style=\"font-family: Arial; font-size: 10pt;\">Lane 1:</strong><span style=\"font-family: Arial; font-size: 10pt;\"> Intact nucleosomes (200 ng total, ~100 ng DNA and ~100 ng histone protein) run on gel.<strong style=\"font-family: Arial; font-size: 10pt;\"> Lane 2:</strong> DNA extracted from nucleosomes (400 ng). Intact nucleosomes migrate much higher than free DNA, thus the DNA resolves at a higher molecular weight when nucleosome-bound. </span><strong style=\"font-family: Arial; font-size: 10pt;\">Note absence of free DNA in Lane 1, indicating all DNA complexed with histones.</strong></td> </tr> <tr valign=\"top\"> <td align=\"center\" valign=\"top\"> <a href=\"#\" target=\"_new\"> <img src=\"#\" alt=\"16-8012 Protein Gel\" width=\"225\" height=\"302\" /></a></td> </tr> <tr> <td align=\"left\" valign=\"top\"><span class=\"Apple-style-span\" style=\"font-family: Arial;\"><span class=\"Apple-style-span\" style=\"font-size: 10pt;\"><strong>Protein Gel Data:</strong> Coomassie stained PAGE gel of proteins in Histone Octamer (H3.3), Human Recombinant (2 μg) to demonstrate the purity of the histones in the preparation. Sizes of molecular weight markers and positions of the core histones (H2A, H2B, H3.3 and H4) are indicated.</span></span></td> </tr> </tbody> </table> </td> </tr> </tbody> </table> </div> </body> </html>","tags":[],"warranty":"","price":{"without_tax":{"formatted":"$215.00","value":215,"currency":"USD"},"tax_label":"Tax"},"detail_messages":"","availability":"","page_title":"Histone Octamer (H3.3), Human Recombinant for Enzyme Activity Screening","cart_url":"https://epicypher-c9.mybigcommerce.com/cart.php","max_purchase_quantity":0,"mpn":null,"upc":null,"options":[],"related_products":[{"id":315,"sku":"15-0312","name":"Histone H3.3, Recombinant Human","url":"https://epicypher-c9.mybigcommerce.com/products/recombinant-proteins/recombinant-histones/histone-h3-3-recombinant-human","availability":"","rating":0,"brand":{"name":null},"category":["Discontinued Products"],"summary":" ","image":null,"images":[],"date_added":"18th Jan 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Pack Size: 50 μg
