SKU: 16-0001
{"num_reviews":0,"url":"https://epicypher-c9.mybigcommerce.com/products/nucleosomes/histone-octamer-recombinant-human","add_this":[{"service":"facebook","annotation":""},{"service":"twitter","annotation":""},{"service":"google","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""}],"gtin":null,"id":128,"can_purchase":true,"meta_description":"Human recombinant histone octamers can be used for chromatin reconstitution experiments, or as substrates for histone modifying enzymes","category":["Nucleosomes","Nucleosomes/Nucleosome Components and Subunits"],"AddThisServiceButtonMeta":"","main_image":null,"add_to_wishlist_url":"/wishlist.php?action=add&product_id=128","custom_fields":[{"id":"11","name":"Pack Size","value":"50 μg"},{"id":"12","name":"Internal Comment","value":"2 lots active, extra included"}],"sku":"16-0001","description":"<div class=\"product-general-info\"> <ul class=\"product-general-info__list-left\"> <li class=\"product-general-info__list-item\"> <strong>Species: </strong>Human </li> <li class=\"product-general-info__list-item\"> <strong>Source: </strong><em>E. coli</em> </li> </ul> <ul class=\"product-general-info__list-right\"> <li class=\"product-general-info__list-item\"> <strong>Tag: </strong>None </li> <li class=\"product-general-info__list-item\"> <strong>Molecular Weight: </strong>108,545 Da </li> </ul> </div> <div class=\"service_accordion product-droppdown\"> <div class=\"container\"> <div id=\"prodAccordion\"> <div id=\"ProductDescription\" class=\"Block Panel current\"> <h3 class=\"sub-title1\">Description</h3> <div class=\"ProductDescriptionContainer product-droppdown__section-description-specific\" style=\"display: block\"> <p> Human histone octamers (two each of histones H2A, H2B, H3 and H4) are made from recombinant histones expressed in <em>E. coli</em>. Octamers are the protein component of nucleosomes, the basic subunit of chromatin. Assembling this product with DNA bearing a 601 Widom sequence (e.g. EpiCypher <a href=\"/products/nucleosomes/nucleosome-assembly-601-sequence-dna-biotinylated\" >18-0005</a >, <a href=\"/products/nucleosomes/nucleosome-components-and-subunits/nucleosome-assembly-601-sequence-dna\" >18-0006</a >) produces a nucleosome. The 601 Widom sequence, originally discovered by Lowary and Widom [1], has high affinity for octamers. For pre-assembled solutions, see <a href=\"/recombinant-nucleosomes-complete-list/\" >our complete list of nucleosome products</a >. </p> </div> </div> </div> <div id=\"prodAccordion\"> <div id=\"ProductDescription\" class=\"Block Panel current\"> <h3 class=\"sub-title1\">Validation Data</h3> <div class=\"ProductDescriptionContainer product-droppdown__section-description-specific\" style=\"display: block\"> <section class=\"image-picker\"> <div class=\"image-picker__left\"> <div class=\"image-picker__main-content_active image-picker__main-content\"> <div class=\"image-picker__header-content\"> <button class=\"image-picker__left-arrow\"> <svg class=\"image-picker__svg-left\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\"> <path d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" /> </svg> </button> <a href=\"/content/images/products/nucleosomes/16-0001-protein-gel-data.png\" target=\"blank\" class=\"image-picker__main-image-link\" ><img alt=\"16-0001-protein-gel-data\" src=\"/content/images/products/nucleosomes/16-0001-protein-gel-data.png\" class=\"image-picker__main-image\" /> <span class=\"image-picker__main-image-caption\" >(Click to enlarge)</span ></a > <button class=\"image-picker__right-arrow\"> <svg class=\"image-picker__svg-right\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\"> <path d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" /> </svg> </button> </div> <p> <span class=\"image-picker__span-content\" ><strong>Figure 1: Protein gel data </strong><br /> Histone Octamer, Recombinant Human (1 µg) run on a SDS-PAGE gel and stained with Coomassie blue to demonstrate the purity of the preparation. The individual histones are indicated. </span> </p> </div> <div class=\"image-picker__main-content\"> <div class=\"image-picker__header-content\"> <button class=\"image-picker__left-arrow\"> <svg class=\"image-picker__svg-left\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\"> <path d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" /> </svg> </button> <a href=\"/content/images/products/nucleosomes/16_0001_wb_web.jpg\" target=\"_blank\" class=\"image-picker__main-image-link\" ><img alt=\"16_0001_wb_web\" src=\"/content/images/products/nucleosomes/16_0001_wb_web.jpg\" class=\"image-picker__main-image\" /> <span class=\"image-picker__main-image-caption\" >(Click to enlarge)</span ></a > <button class=\"image-picker__right-arrow\"> <svg class=\"image-picker__svg-right\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\"> <path d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" /> </svg> </button> </div> <p> <span class=\"image-picker__span-content\" ><strong>Figure 2: Methylation assay data </strong><br /> Histone Octamer, Recombinant Human used in methylation assays with GST-tagged SET domains as indicated. Reactions were loaded onto a PAGE gel and subjected to Western blotting. Top panel: anti-H3K9me2. Bottom panel: anti-histone H3 C-term (EpiCypher <a href=\"/products/antibodies/ptm-specific-antibodies/histone-h3-c-terminal-antibody\" >13-0001</a >). </span> </p> </div> <div class=\"image-picker__main-content\"> <div class=\"image-picker__header-content\"> <button class=\"image-picker__left-arrow\"> <svg class=\"image-picker__svg-left\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\"> <path d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" /> </svg> </button> <a href=\"/content/images/products/nucleosomes/16_0001_pur_web.jpg\" target=\"_blank\" class=\"image-picker__main-image-link\" ><img alt=\"16_0001_pur_web\" src=\"/content/images/products/nucleosomes/16_0001_pur_web.jpg\" class=\"image-picker__main-image\" /> <span class=\"image-picker__main-image-caption\" >(Click to enlarge)</span ></a > <button class=\"image-picker__right-arrow\"> <svg class=\"image-picker__svg-right\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\"> <path d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" /> </svg> </button> </div> <p> <span class=\"image-picker__span-content\" ><strong>Figure 3: Purification data </strong><br /> Chromatogram from gel filtration purification of Histone Octamer, Recombinant Human. Only fractions corresponding to the octamers as indicated were collected and pooled. </span> </p> </div> </div> <aside class=\"image-picker__right\"> <div class=\"image-picker__gallery\"> <img alt=\"16-0001-protein-gel-data\" src=\"/content/images/products/nucleosomes/16-0001-protein-gel-data.png\" width=\"200\" class=\"image-picker__side-image image-picker__side-image_active\" role=\"button\" /> <img alt=\"16_0001_wb_web\" src=\"/content/images/products/nucleosomes/16_0001_wb_web.jpg\" width=\"200\" class=\"image-picker__side-image image-picker__side-image_active\" role=\"button\" /> <img alt=\"16_0001_pur_web\" src=\"/content/images/products/nucleosomes/16_0001_pur_web.jpg\" width=\"200\" class=\"image-picker__side-image image-picker__side-image_active\" role=\"button\" /> </div> </aside> </section> </div> </div> </div> <div id=\"prodAccordion\"> <div id=\"ProductDescription\" class=\"Block Panel\"> <h3 class=\"sub-title1\">Technical Information</h3> <div class=\"ProductDescriptionContainer product-droppdown__section-description\"> <div class=\"product-tech-info\"> <div class=\"product-tech-info__line-item\"> <div class=\"product-tech-info__line-item-left\"> <b>Storage</b> </div> <div class=\"product-tech-info__line-item-right\"> Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid freeze/thaws. </div> </div> <div class=\"product-tech-info__line-item\"> <div class=\"product-tech-info__line-item-left\"> <b>Formulation</b> </div> <div class=\"product-tech-info__line-item-right\"> 10 mM Tris-HCl pH 7.5, 1 mM EDTA, 2 M NaCl, 2 mM DTT, 20% glycerol </div> </div> </div> </div> </div> </div> <div id=\"prodAccordion\"> <div id=\"ProductDescription\" class=\"Block Panel\"> <h3 class=\"sub-title1\">Application Notes</h3> <div class=\"ProductDescriptionContainer product-droppdown__section-description\"> <p> Human recombinant histone octamers can be used for chromatin reconstitution experiments or as substrates for histone modifying enzymes. See <a href=\"https://pubmed.ncbi.nlm.nih.gov/10804500/\" >Luger et al. (1999)</a > for recommended nucleosome reconstitution protocol. Histone octamers should not be stored under conditions where the salt concentration is lower than 800 mM. </p> <p> <strong >Please see lot-specific Technical Datasheets for product concentration.</strong > </p> </div> </div> </div> <div id=\"prodAccordion\"> <div id=\"ProductDescription\" class=\"Block Panel\"> <h3 class=\"sub-title1\">Gene & Protein Information</h3> <div class=\"ProductDescriptionContainer product-droppdown__section-description\"> <div class=\"product-tech-info\"> <div class=\"product-tech-info__line-item\"> <div class=\"product-tech-info__line-item-left\"> <b>UniProt ID</b> </div> <div class=\"product-tech-info__line-item-right\"> <p style=\"margin-bottom: 0\"> H2A - P04908 (alt. names: H2A type 1-B/E, H2A.2, H2A/a, H2A/m) </p> <p style=\"margin-bottom: 0\"> H2B - O60814 (alt. names: H2B K, HIRA-interacting protein 1) </p> <p style=\"margin-bottom: 0\"> H3.1 - P68431 (alt.names: H3, H3/a, H3/b, H3/c, H3/d) </p> <p>H4 - P62805</p> </div> </div> </div> </div> </div> </div> <div id=\"prodAccordion\"> <div id=\"ProductDescription\" class=\"Block Panel\"> <h3 class=\"sub-title1\">References</h3> <div class=\"ProductDescriptionContainer product-droppdown__section-description\"> <b>Background References:</b><br /> [1] Lowary & Widom <em>J. Mol. Biol.</em> (1998). PMID: <a href=\"https://pubmed.ncbi.nlm.nih.gov/9514715/\" title=\"New DNA sequence rules for high affinity binding to histone octamer and sequence-directed nucleosome positioning\" target=\"new\" >9514715</a ><br /> [2] Luger et al. <em>Methods Mol. Biol.</em> (1999). PMID: <a href=\"https://pubmed.ncbi.nlm.nih.gov/10804500/\" title=\"Expression and purification of recombinant histones and nucleosome reconstitution\" target=\"new\" >10804500</a ><br /> </div> </div> </div> <div id=\"prodAccordion\"> <div id=\"ProductDescription\" class=\"Block Panel\"> <h3 class=\"sub-title1\">Documents & Resources</h3> <div class=\"ProductDescriptionContainer product-droppdown__section-description\"> <div class=\"product-documents\"> <a href=\"/content/documents/tds/16-0001.pdf\" target=\"blank\" class=\"product-documents__link\"> <svg version=\"1.1\" id=\"Layer_1\" xmlns=\"http://www.w3.org/2000/svg\" xmlns:xlink=\"http://www.w3.org/1999/xlink\" x=\"0px\" y=\"0px\" viewBox=\"0 0 228 240\" style=\"enable-background: new 0 0 228 240\" xml:space=\"preserve\" class=\"product-documents__icon\"> <g> <path class=\"product-documents__svg-pdf\" d=\"M191.92,68.77l-47.69-47.69c-1.33-1.33-3.12-2.08-5.01-2.08H45.09C41.17,19,38,22.17,38,26.09v184.36 c0,3.92,3.17,7.09,7.09,7.09h141.82c3.92,0,7.09-3.17,7.09-7.09V73.8C194,71.92,193.25,70.1,191.92,68.77z M177.65,77.06h-41.7 v-41.7L177.65,77.06z M178.05,201.59H53.95V34.95h66.92v47.86c0,5.14,4.17,9.31,9.31,9.31h47.86V201.59z\" /> </g> <rect x=\"20\" y=\"112\" class=\"product-documents__svg-background\" width=\"146\" height=\"76\" /> <g> <path class=\"product-documents__svg-pdf\" d=\"M23.83,125.68h22.36c5.29,0,9.41,1.33,12.35,4c2.94,2.67,4.42,6.39,4.42,11.18c0,4.78-1.47,8.51-4.42,11.18 c-2.94,2.67-7.06,4-12.35,4H34.59v18.29H23.83V125.68z M44.81,147.9c5.38,0,8.07-2.32,8.07-6.97c0-2.39-0.67-4.16-2-5.31 c-1.33-1.15-3.36-1.73-6.07-1.73H34.59v14.01H44.81z\" /> <path class=\"product-documents__svg-pdf\" d=\"M69.92,125.68h18.91c5.29,0,9.84,0.97,13.66,2.9c3.82,1.93,6.74,4.72,8.76,8.35 c2.02,3.63,3.04,7.98,3.04,13.04c0,5.06-1,9.42-3,13.08c-2,3.66-4.91,6.45-8.73,8.38c-3.82,1.93-8.4,2.9-13.73,2.9H69.92V125.68z M88.07,165.63c10.35,0,15.52-5.22,15.52-15.66c0-10.4-5.17-15.59-15.52-15.59h-7.38v31.26H88.07z\" /> <path class=\"product-documents__svg-pdf\" d=\"M122.57,125.68h32.84v8.49h-22.22v11.18h20.84v8.49h-20.84v20.49h-10.63V125.68z\" /> </g> </svg> <span class=\"product-documents__info\">Technical Datasheet</span> </a> </div> </div> </div> </div> </div> </div>","tags":[],"warranty":"","price":{"without_tax":{"formatted":"$215.00","value":215,"currency":"USD"},"tax_label":"Tax"},"detail_messages":"","availability":"","page_title":"Recombinant Human Histone Octamer","cart_url":"https://epicypher-c9.mybigcommerce.com/cart.php","max_purchase_quantity":0,"mpn":null,"upc":"50 µg","options":[],"related_products":[{"id":642,"sku":"16-8012","name":"Histone Octamer (H3.3), Human Recombinant","url":"https://epicypher-c9.mybigcommerce.com/products/nucleosomes/histone-octamer-h3-3-human-recombinant","availability":"","rating":0,"brand":{"name":null},"category":["Nucleosomes","Nucleosomes/Nucleosome Components and Subunits"],"summary":" Histone Octamer (H3.3), Human Recombinantt Description: ","image":null,"images":[],"date_added":"18th Jan 2024","pre_order":false,"show_cart_action":true,"has_options":false,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":476,"name":"Pack Size","value":"50 μg"}],"num_reviews":0,"weight":{"formatted":"0.00 Ounces","value":0},"demo":false,"add_to_cart_url":"https://epicypher-c9.mybigcommerce.com/cart.php?action=add&product_id=642","price":{"without_tax":{"currency":"USD","formatted":"$215.00","value":215},"tax_label":"Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=642"},{"id":570,"sku":"16-8018","name":"Histone Octamer (H4 ΔN15), Recombinant Human","url":"https://epicypher-c9.mybigcommerce.com/products/nucleosomes/histone-octamer-h4-n15-recombinant-human","availability":"","rating":0,"brand":{"name":null},"category":["Nucleosomes","Nucleosomes/Nucleosome Components and Subunits"],"summary":"Type a description for this product here...","image":null,"images":[],"date_added":"18th Jan 2024","pre_order":false,"show_cart_action":false,"has_options":false,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":404,"name":"Pack Size","value":"50 µg"}],"num_reviews":0,"weight":{"formatted":"0.00 Ounces","value":0},"demo":false,"price":{"without_tax":{"currency":"USD","formatted":"$215.00","value":215},"tax_label":"Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=570"},{"id":569,"sku":"16-8016","name":"Histone Octamer (H3.1 ΔN32), Recombinant Human","url":"https://epicypher-c9.mybigcommerce.com/products/nucleosomes/histone-octamer-h3-1-n32-recombinant-human","availability":"","rating":0,"brand":{"name":null},"category":["Nucleosomes","Nucleosomes/Nucleosome Components and Subunits"],"summary":"Type a description for this product here...","image":null,"images":[],"date_added":"18th Jan 2024","pre_order":false,"show_cart_action":false,"has_options":false,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":403,"name":"Pack Size","value":"50 µg"}],"num_reviews":0,"weight":{"formatted":"0.00 Ounces","value":0},"demo":false,"price":{"without_tax":{"currency":"USD","formatted":"$215.00","value":215},"tax_label":"Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=569"},{"id":167,"sku":"15-0301","name":"Histone H2A, Recombinant Human","url":"https://epicypher-c9.mybigcommerce.com/products/recombinant-proteins/recombinant-histones/histone-h2a-recombinant-human","availability":"","rating":0,"brand":{"name":null},"category":["Recombinant Proteins/Recombinant Histones"],"summary":" ","image":null,"images":[],"date_added":"18th Jan 2024","pre_order":false,"show_cart_action":true,"has_options":false,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":52,"name":"Pack Size","value":"100 μg"}],"num_reviews":0,"weight":{"formatted":"0.01 Ounces","value":0.01},"demo":false,"add_to_cart_url":"https://epicypher-c9.mybigcommerce.com/cart.php?action=add&product_id=167","price":{"without_tax":{"currency":"USD","formatted":"$115.00","value":115},"tax_label":"Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=167"},{"id":315,"sku":"15-0312","name":"Histone H3.3, Recombinant Human","url":"https://epicypher-c9.mybigcommerce.com/products/recombinant-proteins/recombinant-histones/histone-h3-3-recombinant-human","availability":"","rating":0,"brand":{"name":null},"category":["Discontinued Products"],"summary":" ","image":null,"images":[],"date_added":"18th Jan 2024","pre_order":false,"show_cart_action":true,"has_options":false,"stock_level":null,"low_stock_level":null,"qty_in_cart":0,"custom_fields":[{"id":176,"name":"Pack Size","value":"100 μg"},{"id":177,"name":"Internal Comment","value":"extra in top right of Psylocke, BULK incl."}],"num_reviews":0,"weight":{"formatted":"0.01 Ounces","value":0.01},"demo":false,"add_to_cart_url":"https://epicypher-c9.mybigcommerce.com/cart.php?action=add&product_id=315","price":{"without_tax":{"currency":"USD","formatted":"$115.00","value":115},"tax_label":"Tax"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=315"}],"shipping_messages":[],"rating":0,"reviews":{"messages":[],"captcha":"6LdWf8gSAAAAAI83aRectJhbwidegZKk8PzWBltH","total":0,"show_review_email":true,"recaptcha":{"enabled":1,"public_key":"6LdWf8gSAAAAAI83aRectJhbwidegZKk8PzWBltH","markup":"<div class=\"g-recaptcha\" data-sitekey=\"6LcjX0sbAAAAACp92-MNpx66FT4pbIWh-FTDmkkz\"></div><br/>"}},"bulk_discount_rates":[],"meta_keywords":"Recombinant Human Histone Octamer, histone octamer, recombinant nucleosome, chromatin, recombinant histone, recombinant chromatin, nucleosome reconstitution","show_quantity_input":1,"title":"Histone Octamer, Recombinant Human","gift_wrapping_available":false,"min_purchase_quantity":0,"customizations":[],"images":[]}
Pack Size: 50 μg
