SKU: 16-0322
{"num_reviews":0,"url":"https://epicypher-c9.mybigcommerce.com/products/nucleosomes/modified-designer-nucleosomes-dnucs/nucleosome-recombinant-human-h3k36me1-dnuc-biotinylated","add_this":[{"service":"facebook","annotation":""},{"service":"twitter","annotation":""},{"service":"google","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""}],"gtin":null,"id":352,"can_purchase":true,"meta_description":"H3K36Me1 Recombinant Nucleosome (dNuc) for Enzyme Screening Assays","category":["Nucleosomes/Modified Designer Nucleosomes (dNucs™)"],"AddThisServiceButtonMeta":"","main_image":null,"add_to_wishlist_url":"/wishlist.php?action=add&product_id=352","custom_fields":[{"id":"202","name":"Pack Size","value":"50 μg"}],"sku":"16-0322","description":"<div class=\"product-general-info\"> <ul class=\"product-general-info__list-left\"> <li class=\"product-general-info__list-item\"> <strong>Species: </strong>Human </li> <li class=\"product-general-info__list-item\"> <strong>Source: </strong><i>E. coli</i> & synthetic DNA </li> </ul> <ul class=\"product-general-info__list-right\"> <li class=\"product-general-info__list-item\"> <strong>Tag: </strong>Biotinylated </li> <li class=\"product-general-info__list-item\"> <strong>Molecular Weight: </strong>199,770.3 Da </li> </ul> </div> <div class=\"service_accordion product-droppdown\"> <div class=\"container\"> <div id=\"prodAccordion\"> <div id=\"ProductDescription\" class=\"Block Panel current\"> <h3 class=\"sub-title1\">Description</h3> <div class=\"ProductDescriptionContainer product-droppdown__section-description-specific\"> <p> Designer mononucleosomes (dNucs) consist of 147 base pairs of DNA wrapped around an octamer core of histone proteins (two each of H2A, H2B, H3.1, H4) to form a nucleosome, the basic repeating unit of chromatin. The 147 bp 601 sequence, identified by Lowary and Widom [1], has high affinity for histone octamers and is useful for nucleosome assembly. H3K36me1 dNuc contains monomethyl-lysine at position 36 on histone H3.1. The DNA in this nucleosome contains a 5’biotin-TEG group. </p> </div> </div> </div> <div id=\"prodAccordion\"> <div id=\"ProductDescription\" class=\"Block Panel current\"> <h3 class=\"sub-title1\">Validation Data</h3> <div class=\"ProductDescriptionContainer product-droppdown__section-description-specific\"> <section class=\"image-picker\"> <div class=\"image-picker__left\"> <div class=\"image-picker__main-content_active image-picker__main-content\"> <div class=\"image-picker__header-content\"> <button class=\"image-picker__left-arrow\"> <svg class=\"image-picker__svg-left\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\"> <path d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" /> </svg> </button> <a href=\"/content/images/products/nucleosomes/16-0322-western-blot-data.jpeg\" target=\"_blank\" class=\"image-picker__main-image-link\" ><img alt=\"16-0322-dna-gel-data\" src=\"/content/images/products/nucleosomes/16-0322-western-blot-data.jpeg\" class=\"image-picker__main-image\" /> <span class=\"image-picker__main-image-caption\" >(Click to enlarge)</span ></a > <button class=\"image-picker__right-arrow\"> <svg class=\"image-picker__svg-right\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\"> <path d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" /> </svg> </button> </div> <p> <span class=\"image-picker__span-content\" ><strong>Figure 1: Western blot data </strong><br /> Western Analysis of H3K36me1 dNuc. <strong>Top Panel:</strong> Unmodified (EpiCypher <a href=\"https://www.epicypher.com/products/nucleosomes/mononucleosomes-recombinant-human-biotinylated\" >16-0006</a >; Lane 1) and H3K36me1 nucleosomes (Lane 2) were probed with an anti-H3K36me1 antibody and analyzed via ECL readout. Only the H3K36me1 sample produced a detectable signal. <strong>Bottom Panel:</strong> Detail from Coomassie stained gel showing unmodified (Lane 1) and H3K36me1 nucleosomes (Lane 2). </span> </p> </div> <div class=\"image-picker__main-content\"> <div class=\"image-picker__header-content\"> <button class=\"image-picker__left-arrow\"> <svg class=\"image-picker__svg-left\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\"> <path d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" /> </svg> </button> <a href=\"/content/images/products/nucleosomes/16-0322-mass-spec-data.jpeg\" target=\"_blank\" class=\"image-picker__main-image-link\" ><img alt=\"16-0322-protein-gel-data-data\" src=\"/content/images/products/nucleosomes/16-0322-mass-spec-data.jpeg\" class=\"image-picker__main-image\" /> <span class=\"image-picker__main-image-caption\" >(Click to enlarge)</span ></a > <button class=\"image-picker__right-arrow\"> <svg class=\"image-picker__svg-right\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\"> <path d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" /> </svg> </button> </div> <p> <span class=\"image-picker__span-content\" ><strong>Figure 2: Mass spec data </strong><br /> Synthetic H3K36me1 histone analyzed by high resolution mass spectrometry. Expected mass = 15,286.9 Da. Determined mass = 15,286.91 Da. </span> </p> </div> <div class=\"image-picker__main-content\"> <div class=\"image-picker__header-content\"> <button class=\"image-picker__left-arrow\"> <svg class=\"image-picker__svg-left\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\"> <path d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" /> </svg> </button> <a href=\"/content/images/products/nucleosomes/16-0322-protein-gel-data.jpeg\" target=\"_blank\" class=\"image-picker__main-image-link\"> <img alt=\"16-0322-protein-gel-data-data\" src=\"/content/images/products/nucleosomes/16-0322-protein-gel-data.jpeg\" class=\"image-picker__main-image\" /> <span class=\"image-picker__main-image-caption\" >(Click to enlarge)</span > </a> <button class=\"image-picker__right-arrow\"> <svg class=\"image-picker__svg-right\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\"> <path d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" /> </svg> </button> </div> <p> <span class=\"image-picker__span-content\"> <strong>Figure 3: Protein gel data </strong><br /> Coomassie stained SDS-PAGE gel of proteins in H3K36me1 dNuc (1 µg) demonstrates the purity of histones in the preparation. Sizes of molecular weight markers and positions of the core histones (H2A, H2B, H3K36me1 and H4) are indicated. </span> </p> </div> <div class=\"image-picker__main-content\"> <div class=\"image-picker__header-content\"> <button class=\"image-picker__left-arrow\"> <svg class=\"image-picker__svg-left\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\"> <path d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" /> </svg> </button> <a href=\"/content/images/products/nucleosomes/16-0322-dna-gel-data.jpeg\" target=\"_blank\" class=\"image-picker__main-image-link\" ><img alt=\"16-0322-dna-gel-data\" src=\"/content/images/products/nucleosomes/16-0322-dna-gel-data.jpeg\" class=\"image-picker__main-image\" /> <span class=\"image-picker__main-image-caption\" >(Click to enlarge)</span ></a > <button class=\"image-picker__right-arrow\"> <svg class=\"image-picker__svg-right\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\"> <path d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" /> </svg> </button> </div> <p> <span class=\"image-picker__span-content\" ><strong>Figure 4: DNA gel data </strong><br /> H3K36me1 dNuc resolved via native PAGE and stained with ethidium bromide to visualize DNA. <strong>Lane 1:</strong> Free DNA (EpiCypher <a href=\"/products/nucleosomes/nucleosome-assembly-601-sequence-dna-biotinylated\" target=\"_blank\" >18-0005</a >; 100 ng). <strong>Lane 2:</strong> Intact H3K36me1 nucleosomes (400 ng). </span> </p> </div> </div> <aside class=\"image-picker__right\"> <div class=\"image-picker__gallery\"> <img alt=\"16-0322-western-blot-data\" src=\"/content/images/products/nucleosomes/16-0322-western-blot-data.jpeg\" width=\"200\" class=\"image-picker__side-image image-picker__side-image_active\" role=\"button\" /> <img alt=\"16-0322-mass-spec-data\" src=\"/content/images/products/nucleosomes/16-0322-mass-spec-data.jpeg\" class=\"image-picker__side-image\" role=\"button\" /> <img alt=\"16-0322-protein-gel-data-data\" src=\"/content/images/products/nucleosomes/16-0322-protein-gel-data.jpeg\" class=\"image-picker__side-image\" role=\"button\" /> <img alt=\"16-0322-dna-gel-data-data\" src=\"/content/images/products/nucleosomes/16-0322-dna-gel-data.jpeg\" class=\"image-picker__side-image\" role=\"button\" /> </div> </aside> </section> </div> </div> </div> <div id=\"prodAccordion\"> <div id=\"ProductDescription\" class=\"Block Panel\"> <h3 class=\"sub-title1\">Technical Information</h3> <div class=\"ProductDescriptionContainer product-droppdown__section-description\"> <div class=\"product-tech-info\"> <div class=\"product-tech-info__line-item\"> <div class=\"product-tech-info__line-item-left\"> <b>Storage</b> </div> <div class=\"product-tech-info__line-item-right\"> Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid freeze/thaws. </div> </div> <div class=\"product-tech-info__line-item\"> <div class=\"product-tech-info__line-item-left\"> <b>Formulation</b> </div> <div class=\"product-tech-info__line-item-right\"> 10 mM Tris HCl pH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM DTT, 20% glycerol (27.3 µg protein, 50 µg DNA + protein) </div> </div> </div> </div> </div> </div> <div id=\"prodAccordion\"> <div id=\"ProductDescription\" class=\"Block Panel\"> <h3 class=\"sub-title1\">Application Notes</h3> <div class=\"ProductDescriptionContainer product-droppdown__section-description\"> <p> H3K36me1 dNuc is highly purified and suitable for a variety of applications, including use as a substrate in enzyme assays, high-throughput screening and inhibitor testing, chromatin binding studies, protein-protein interaction assays, structural studies, and in effector protein binding experiments. </p> </div> </div> </div> <div id=\"prodAccordion\"> <div id=\"ProductDescription\" class=\"Block Panel\"> <h3 class=\"sub-title1\">Gene & Protein Information</h3> <div class=\"ProductDescriptionContainer product-droppdown__section-description\"> <div class=\"product-tech-info\"> <div class=\"product-tech-info__line-item\"> <div class=\"product-tech-info__line-item-left\"> <b>UniProt ID</b> </div> <div class=\"product-tech-info__line-item-right\"> H2A - P04908 (alt. names: H2A type 1-B/E, H2A.2, H2A/a, H2A/m)<br /> H2B - O60814 (alt. names: H2B K, HIRA-interacting protein 1)<br /> H3.1 - P68431 (alt. names: H3, H3/a, H3/b, H3/c, H3/d)<br /> H4 - P62805 </div> </div> </div> </div> </div> </div> <div id=\"prodAccordion\"> <div id=\"ProductDescription\" class=\"Block Panel\"> <h3 class=\"sub-title1\">References</h3> <div class=\"ProductDescriptionContainer product-droppdown__section-description\"> <strong>Background References:</strong> <br /> [1] Lowary and Widom <em>J. Mol. Biol.</em> (1998). PMID: <a href=\"https://pubmed.ncbi.nlm.nih.gov/9514715/\" target=\"_blank\" title=\"New DNA sequence rules for high affinity binding to histone octamer and sequence-directed nucleosome positioning\" >9514715</a ><br /> </div> </div> </div> <div id=\"prodAccordion\"> <div id=\"ProductDescription\" class=\"Block Panel\"> <h3 class=\"sub-title1\">Documents & Resources</h3> <div class=\"ProductDescriptionContainer product-droppdown__section-description\"> <div class=\"product-documents\"> <a href=\"/content/documents/tds/16-0322.pdf\" target=\"_blank\" class=\"product-documents__link\"> <svg version=\"1.1\" id=\"Layer_1\" xmlns=\"http://www.w3.org/2000/svg\" xmlns:xlink=\"http://www.w3.org/1999/xlink\" x=\"0px\" y=\"0px\" viewBox=\"0 0 228 240\" style=\"enable-background: new 0 0 228 240\" xml:space=\"preserve\" class=\"product-documents__icon\" alt=\"16-0322 Datasheet\"> <g> <path class=\"product-documents__svg-pdf\" d=\"M191.92,68.77l-47.69-47.69c-1.33-1.33-3.12-2.08-5.01-2.08H45.09C41.17,19,38,22.17,38,26.09v184.36 c0,3.92,3.17,7.09,7.09,7.09h141.82c3.92,0,7.09-3.17,7.09-7.09V73.8C194,71.92,193.25,70.1,191.92,68.77z M177.65,77.06h-41.7 v-41.7L177.65,77.06z M178.05,201.59H53.95V34.95h66.92v47.86c0,5.14,4.17,9.31,9.31,9.31h47.86V201.59z\" /> </g> <rect x=\"20\" y=\"112\" class=\"product-documents__svg-background\" width=\"146\" height=\"76\" /> <g> <path class=\"product-documents__svg-pdf\" d=\"M23.83,125.68h22.36c5.29,0,9.41,1.33,12.35,4c2.94,2.67,4.42,6.39,4.42,11.18c0,4.78-1.47,8.51-4.42,11.18 c-2.94,2.67-7.06,4-12.35,4H34.59v18.29H23.83V125.68z M44.81,147.9c5.38,0,8.07-2.32,8.07-6.97c0-2.39-0.67-4.16-2-5.31 c-1.33-1.15-3.36-1.73-6.07-1.73H34.59v14.01H44.81z\" /> <path class=\"product-documents__svg-pdf\" d=\"M69.92,125.68h18.91c5.29,0,9.84,0.97,13.66,2.9c3.82,1.93,6.74,4.72,8.76,8.35 c2.02,3.63,3.04,7.98,3.04,13.04c0,5.06-1,9.42-3,13.08c-2,3.66-4.91,6.45-8.73,8.38c-3.82,1.93-8.4,2.9-13.73,2.9H69.92V125.68z M88.07,165.63c10.35,0,15.52-5.22,15.52-15.66c0-10.4-5.17-15.59-15.52-15.59h-7.38v31.26H88.07z\" /> <path class=\"product-documents__svg-pdf\" d=\"M122.57,125.68h32.84v8.49h-22.22v11.18h20.84v8.49h-20.84v20.49h-10.63V125.68z\" /> </g> </svg> <span class=\"product-documents__info\">Technical Datasheet</span> </a> </div> </div> </div> </div> </div> </div>","tags":[],"warranty":"","price":{"without_tax":{"formatted":"$575.00","value":575,"currency":"USD"},"tax_label":"Tax"},"detail_messages":"","availability":"","page_title":"H3K36Me1 Recombinant Nucleosome (dNuc) for Enzyme Screening Assays","cart_url":"https://epicypher-c9.mybigcommerce.com/cart.php","max_purchase_quantity":0,"mpn":null,"upc":null,"options":[],"related_products":[{"id":693,"sku":"16-0379","name":"Nucleosome, Recombinant Human, H3R8me1 dNuc, 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Pack Size: 50 μg
